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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Animal Metabolism-Agricultural Chemicals Research » Research » Publications at this Location » Publication #325916
Title: In-vitro synthesis of drug metabolites and their screening/characterization using liquid chromatography-mass spectrometry (LC-MS)

Author
item CHAKRABARTY, SHUBHASHIS - Orise Fellow
item Shelver, Weilin
item Thompson, Andrew
item Smith, David

Submitted to: American Society for Mass Spectrometry
Publication Type: Abstract Only
Publication Acceptance Date: 2/3/2016
Publication Date: 6/5/2016
Citation: Chakrabarty, S., Shelver, W.L., Thompson, A.R., Smith, D.J. 2016. In-vitro synthesis of drug metabolites and their screening/characterization using liquid chromatography-mass spectrometry (LC-MS). In: Proceedings of the 64th Conference on Mass Spectrometry and Allied Topics. June 5-9, 2016. San Antonio, TX. p. 99.

Interpretive Summary: Drug metabolism is a biochemical process by which drugs and xenobiotics are chemically modified to metabolites, primarily by liver enzymes. Metabolites may sometimes affect cellular therapeutic or toxicological processes, therefore knowledge of metabolic processes is essential for understanding drug or chemical actions in cellular systems.1 However, low recovery of metabolites from biological matrices makes identification and study difficult. Conventional chemical synthesis of metabolites is difficult, time consuming and expensive, but is often necessary.2 Here we demonstrate, the in-vitro synthesis and structural elucidation of metabolites of flunixin, a non-steroidal anti-inflammatory agent, and dicoumarol, a fungal anticoagulant using LC-MS/MS.

Technical Abstract: Drug metabolism is a biochemical process by which drugs and xenobiotics are chemically modified to metabolites, primarily by liver enzymes. Metabolites may sometimes affect cellular therapeutic or toxicological processes, therefore knowledge of metabolic processes is essential for understanding drug or chemical actions in cellular systems.1 However, low recovery of metabolites from biological matrices makes identification and study difficult. Conventional chemical synthesis of metabolites is difficult, time consuming and expensive, but is often necessary.2 Here we demonstrate, the in-vitro synthesis and structural elucidation of metabolites of flunixin, a non-steroidal anti-inflammatory agent, and dicoumarol, a fungal anticoagulant using LC-MS/MS.