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Title: CHARACTERIZATION OF EXCRETORY-SECRETORY (ES) PRODUCTS FROM HAEMONCHUS CONTORTUS LARVAE CULTURED IN VITRO TO THE FOURTH STAGE

Author
item GAMBLE HOWARD R - 1265-20-00
item MANSFIELD LINDA - 1265-20-00

Submitted to: American Association of Veterinary Parasitologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/18/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Haemonchus contortus larvae developed in vitro from third to fourth stage larvae in 48-96 hours. Metabolic activity, as measured by messenger RNA production and in vitro translation products, increased following stimulation of infective stages with CO2. However, secretion of significant amounts of protein into culture media and larval feeding did not occur until larvae had molted to the fourth stage. Fourth stage larvae secreted several enzymes into culture including a metalloprotease, an acid phosphohydrolase, a cathepsin C-like enzyme, a phospholipase C- like enzyme and an N-acetyl-b-D-glucosaminidase. Secreted products from fourth stage larvae had antigenic homologies with ES products produced during the second molt and with proteins and glycoproteins extracted from third and fourth stage larvae. Further, these ES products were recognized by sera from sheep infected with H. contortus. The enzymes identified here might serve as targets for immune or chemical control of trichostrongyle infections.