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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #324472

Research Project: Genetic Improvement of Wheat and Barley for Resistance to Biotic and Abiotic Stresses

Location: Wheat Health, Genetics, and Quality Research

Title: Cereal cyst nematode screening in locally adapted spring wheat (Triticum aestivum L.) germplasm of the Pacific Northwest, 2015

Author
item Manning-thompson, Y.
item Thompson, A.
item Paulitz, Timothy
item Smiley, R
item Garland-campbell, Kimberly

Submitted to: Plant Disease Management Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/12/2016
Publication Date: 3/7/2016
Citation: Manning-Thompson, Y., Thompson, A., Paulitz, T.C., Smiley, R., Garland Campbell, K.A. 2016. Cereal cyst nematode screening in locally adapted spring wheat (Triticum aestivum L.) germplasm of the Pacific Northwest, 2015. Plant Disease Management Reports. 10:N003.

Interpretive Summary: The problem is that cyst nematodes are a yield damaging pest to wheat in the Pacific Northwest. Our research was conducted to determine if adapted varieties and breeding lines exhibited differential resistance to cereal cyst nematodes in field screening trials conducted in Washington from 2013-2015. We discovered that small number of spring wheat cultivars did exhibit better resistance to cyst nematodes and could be useful in breeding wheat for resistance to this disease.

Technical Abstract: Field screenings were performed to determine if there is resistance to Heterodera filipjevi in locally adapted wheat germplasm which could be introgressed into new WA wheat varieties. A field naturally infested with Heterodera filipjevi located in Colton, WA, was selected for this experiment. Cultivars and breeding lines from the Western Spring Regional Nursery and Washington State Extension Trials, 2013 and 2014 and 2015 respectively, were screened. Experimental site was kept in a two year rotation of spring wheat and barley for upkeep of disease pressure. A Wintersteiger headrow planter was used to plant plots. Fertilizer and chemical controls were applied according to conventional protocols. Seeds were sown at the rate of ? lb/A in four-row plots, an average length of 1 m long, with a .35 m spacing between rows. Head rows consisted of germplasm planted in two rows, side by side alongside two rows of a susceptible check, “ALPOWA”. The experimental design was a replicated complete block design with five replications in 2013 and three replication in 2014 and 2015. Samples (approximately .3 m long) were collected 45-60 days after planting at the Zadok 45-55 growth stage. Five plants from each sample were used as a pooled sample and the number of white female cysts visible on the roots were assessed using a 0-5 rating scale. Breeding line UC1741 and cultivars SY Steelhead and Chara had the lowest disease rating. Breeding line WA 8189 had the highest disease rating and was statistical similar to entries LCS-Buck Pronto, UI Winchester, JD, WB-1035CL+, 11SB0096, Diva, WB-9879CLP, Jefferson, Patwin 515, IDO862E, M12001, M12003, IDO851, Bullseye, and BR7030.