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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #323879

Research Project: NEW AND EMERGING VIRAL AND BACTERIAL DISEASES OF ORNAMENTAL PLANTS: DETECTION, IDENTIFICATION, AND CHARACTERIZATION

Location: Floral and Nursery Plants Research

Title: 2015 nationwide survey revealed Barley stripe mosaic virus in Korean barley fields

Author
item Lim, Hyojin - Chungnam National University
item Seo, Eun-young - Chungnam National University
item Kim, Hyun-seung - Chungnam National University
item Kim, Jung-kyu - Chungnam National University
item Park, Chan-hwan - Chungnam National University
item Gong, Jun-su - Chungnam National University
item Him, Ik-hyun - Chungnam National University
item Han, Ang-huk - Chungnam National University
item Kilcrease, James - Orise Fellow
item Tsuchiya, Kenichi - Kyushu University
item Hammond, John
item Lim, Hyoun-sub - Chungnam National University

Submitted to: Journal of Faculty of Agriculture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/16/2015
Publication Date: 1/11/2016
Citation: Lim, H., Seo, E., Kim, H., Kim, J., Park, C., Gong, J., Him, I., Han, A., Kilcrease, J.P., Tsuchiya, K., Hammond, J., Lim, H. 2016. 2015 nationwide survey revealed Barley stripe mosaic virus in Korean barley fields. Journal of Faculty of Agriculture. 61:71-77.

Interpretive Summary: Barley production is expanding in Korea due to increasing interest in health foods; barley viruses cause losses of both yield and quality, and are therefore of concern to farmers. There has been an increase in the proportion of seed that is imported to Korea, with the risk of introduction of new seed-transmitted viruses affecting the crop. Seed-transmission of viruses results in establishment of infection early in the crop cycle, and potentially rapid spread throughout the growing season, resulting in lower crop yield and quality. A nationwide survey was carried out in Korea to detect seed-transmitted viruses in barley, and Barley stripe mosaic virus (BSMV) was detected at 20 locations. Some of the newly-detected isolates were shown to have differences from previously-characterized isolates, but were most closely related to isolates from the USA. These results will serve as baseline information against which to compare future detection of BSMV in Korea, and the methods developed will be useful to evaluate and document virus prevention and control measures.

Technical Abstract: A seed-transmitted virus has consistently caused significant economic damage to barley crops in Korea in recent years, and may be increasing because many farmers save seed for replanting. Because some barley seed is imported, there is the potential for introduction of new seed-transmitted viruses, causing diseases which may spread. Barley cultivation in South Korea is expanding nationwide due to the increasing popularity of health foods, so both production and quality of barley grain is important. Although Barley stripe mosaic virus (BSMV) has been reported previously as a small percentage of all barley viruses present in Korea, increases in imports of barley seed may lead to increased occurrence of this seed-transmitted virus. We therefore investigated the virus status of barley crops around Iksan and Wanju. On several newly cultivated barley farms, we observed areas showing symptoms typical of BSMV, and confirmed BSMV infection in 24% of the samples examined. In order to understand the occurrence and seed transmission of Korean BSMV isolates, we examined sequence variation within the Triple Gene Block proteins, and subcellular localization of two of these proteins. The newly identified Korean BSMV isolates show low sequence variability and high sequence homology to previously reported US isolates. With these results, we expect to confirm distribution of barley viruses and possible emerging viruses, which will serve as base line data to document virus prevention and control measures.