Location: Subtropical Horticulture ResearchTitle: A genetic map and germplasm diversity estimation of Mangifera indica (mango) with SNPs Author
|Amir, Sherman - Volcani Center (ARO)|
|Yuval, Cohen - Volcani Center (ARO)|
|Ophir, Ron - Volcani Center (ARO)|
|Groh, Amy - Mars, Inc|
|Rahaman, Jordan - Mars, Inc|
|Sanchez, Paola - Mars, Inc|
|Dillon, Natalie - Queensland Department Of Primary Industries & Fisheries|
|Bally, Ian - Queensland Department Of Primary Industries & Fisheries|
|Innes, David - Queensland Department Of Primary Industries & Fisheries|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/13/2015
Publication Date: 1/11/2016
Citation: Kuhn, D.N., Amir, S., Yuval, C., Ophir, R., Groh, A., Rahaman, J., Sanchez, P., Dillon, N., Bally, I., Innes, D. 2016. A genetic map and germplasm diversity estimation of Mangifera indica (mango) with SNPs. Meeting Abstract. 3:2.
Interpretive Summary: The Subtropical Horticulture Research Station in Miami, Florida houses living collections of a diverse variety of tropical and subtropical fruit trees, such as avocado, cacao, lychee, and mango, among others. These germplasm collections are resources for plant breeders, scientists studying fruit trees and nurseries hoping to expand their holdings to whom we distribute living plant material through our online request system (Germplasm Resource Information Network, http://www.ars-grin.gov/). We have developed single-nucleotide polymorphism (SNP) molecular genetic markers for mango to be able to create a mango genetic map and assess the genetic diversity in our mango germplasm collection. The information presented in the paper is a significant advance in curating the germplasm collection to provide users, breeders and stakeholders with the highest quality, disease free germplasm material.
Technical Abstract: Mango (Mangifera indica) is often referred to as the “King of Fruits”. As the first steps in developing a mango genomics project, we genotyped 582 individuals comprising six mapping populations with 1054 SNP markers. The resulting consensus map had 20 linkage groups defined by 726 SNP markers with 67 SNPs as the maximum number for one linkage group and 20 SNPs the minimum number. Although mango has been described as an allotetraploid with 40 chromosomes (n = 10), all SNPs showed disomic inheritance and Mendelian segregation patterns of a diploid with n = 20. SNP markers (384) that were evenly distributed across the mango genome as defined by map position were used to genotype ~800 individuals including other Mangifera species from several germplasm collections in a first round of germplasm genetic diversity estimation. Clustering and STRUCTURE analysis of the germplasm data will be presented.