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ARS Home » Research » Publications at this Location » Publication #323431
Title: The Tgm9-induced indexed insertional mutant collection to conduct community-based reverse genetics studies in soybean

Author
item BHATTACHARYA, MADAN - Iowa State University
item BAUMBACH, JORDAN - Iowa State University
item O'MALLEY, RONON - Salk Institute
item Sandhu, Devinder

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/9/2016
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Until now, functional analyses of soybean genes have been very arduous because of the lack of a rapid transformation procedure. Recently identified the active endogenous type II transposable element, Tgm9, excises from insertion sites and restores wild-type phenotypes. Thus, this element provides a great promise in cloning soybean genes bypassing challenges associated with soybean transformation. By applying a novel high-throughput technology, we determined Tgm9-insertion sites in 5,184 lines. These Tgm9 insertions are found in all twenty soybean chromosomes showing preferential re-insertion into gene-rich chromosomal arms as compared to gene-poor centromeric regions. Among the 5,184 mutants, 1,542 represent potential Tgm9-induced gene-disruption mutants at a rate of 50 to 106 per chromosome. Considering that only 16% of the soybean genome contains genes, this study establishes that a Tgm9 mutagenesis population is enriched for potential gene-disruption mutants making Tgm9 an important resource for functional analysis of soybean genes.