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ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Genetics and Sustainable Agriculture Research » Research » Publications at this Location » Publication #323406
Title: RNA interference for functional genomics and improvement of cotton (Gossypium species)

Author
item ABDURAKHMONOV, IBROKHIM - Uzbekistan Academy Of Sciences
item AYUBOV, MIRZAKAMOL - Uzbekistan Academy Of Sciences
item UBAYDULLAEVA, KHURSHIDA - Uzbekistan Academy Of Sciences
item BURIEV, BURIEV - Uzbekistan Academy Of Sciences
item SHERMATOV, SHUKHRAT - Uzbekistan Academy Of Sciences
item RUZIBOEV, HAYDARALI - Uzbekistan Academy Of Sciences
item SHAPULATOV, UMID - Uzbekistan Academy Of Sciences
item Saha, Sukumar
item Ulloa, Mauricio
item Yu, John
item Percy, Richard
item DEVOR, ERIC - University Of Iowa
item GOVIND, SHARMA - Alabama A & M University
item SRIPATHI, VENKATESWARA - Alabama A & M University
item KUMPATLA, SIVA - Dow Agro Sciences
item VAN DE KROLL, ALEXSANDER - Wageningen University
item HAKE, KATER - Cotton, Inc
item KHAMIDOV, KHAKINJON - Uzbekistan Academy Of Sciences
item SALIKHOV, SHAVKAT - Uzbekistan Academy Of Sciences
item Jenkins, Johnie
item ABDUKARIMOV, ABDUSATTOR - Uzbekistan Academy Of Sciences
item PEPPER, ALAN - Texas A&M University

Submitted to: Frontiers in Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/5/2016
Publication Date: 2/22/2016
Publication URL: https://handle.nal.usda.gov/10113/62572
Citation: Abdurakhmonov, I.Y., Ayubov, M., Ubaydullaeva, K.A., Buriev, B.T., Shermatov, S.E., Ruziboev, H., Shapulatov, U.M., Saha, S., Ulloa, M., Yu, J., Percy, R.G., Devor, E.J., Govind, S.C., Sripathi, V.R., Kumpatla, S.P., Van De Kroll, A., Hake, K.D., Khamidov, K., Salikhov, S.I., Jenkins, J.N., Abdukarimov, A., Pepper, A.E. 2016. RNA interference for functional genomics and improvement of cotton (Gossypium species). Frontiers in Plant Science. 7:202.

Interpretive Summary: RNA interference (RNAi), a powerful new technology, provides a tool to block gene function by inserting short sequences of ribonucleic acid (RNA) that match part of the target gene’s sequence of interest and thus block the formation of protein and gene function. This article provides a comprehensive review on the application of RNAi technology in the discovery of function and biological roles of many key cotton genes involved in fiber development, fertility and somatic embryogenesis, resistance to important biotic and abiotic stresses, and oil and seed quality improvements as well as improving the key agronomic traits including yield and maturity. This paper also presents some ongoing efforts in developing commercially viable cotton lines using targeted applications of RNAi technologies and small- or large-scale field trials of RNAi cotton lines, The paper also addressed several safety risk issues of RNAi cotton lines.

Technical Abstract: RNA interference (RNAi), is a powerful new technology in the discovery of genetic sequence functions, and has become a valuable tool for functional genomics of cotton (Gossypium ssp.). The rapid adoption of RNAi has replaced previous antisense technology. RNAi has aided in the discovery of function and biological roles of many key cotton genes involved in fiber development, fertility and somatic embryogenesis, resistance to important biotic and abiotic stresses, and oil and seed quality improvements as well as the key agronomic traits including yield and maturity. Here, we have comparatively reviewed seminal research efforts in previously used antisense approaches and currently applied breakthrough RNAi studies in cotton, briefly describing developed RNAi methodologies, achievements, and limitations in functional characterizations of cotton genes as well as efforts in the development of RNAi-based cotton cultivars, their safety and risk assessment, small and large-scale field trials, and commercialization intentions.