|XIANG, YANG - University Of California
Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 10/22/2015
Publication Date: 10/29/2015
Citation: Lin, H., Xiang, Y. 2015. Functional characterization of the role of the PilG in Xylella fastidiosa. CDFA Pierce's Disease Control Program Research Symposium, December 18, 2015, Sacramento, California. p.245.
Technical Abstract: Type IV pili of Xylella fastidiosa are regulated by pilG, a chemotaxis regulator in the Pil-Chp operon involving signal transduction pathways. To elucidate the role of pilG in twitching motility and pathogenicity of X. fastidiosa, phenotypes of wild type, a pilG-mutant, and a complementary strain were characterized. While all tested strains had similar growth curves in vitro, X. fastidiosa wild type and complementary Xf'pilG-C strain showed typical twitching motility in microfluidic flow chambers, whereas mutant Xf'pilG exhibited a twitching defective phenotype. Greenhouse experiment further revealed that Pierce’s disease (PD) symptoms were significantly reduced in grapevines inoculated with Xf'pilG, whereas grapevines inoculated with X. fastidiosa wild type and Xf'pilG-C developed typical PD symptoms. These results demonstrate that pilG of X. fastidiosa is required for twitching motility and full virulence.