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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #322947

Research Project: IMMUNOLOGY AND INTERVENTION STRATEGIES FOR JOHNE'S DISEASE

Location: Infectious Bacterial Diseases Research

Title: Gamma-delta T cell responses in subclinical and clinical stages of Bovine Mycobacterium Avium Paratuberculosis infection

Author
item Albarrak, Saleh - Iowa State University
item Waters, Wade
item Stabel, Judith
item Hostetter, J - Iowa State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/28/2015
Publication Date: 10/20/2015
Citation: Albarrak, S., Waters, W.R., Stabel, J.R., Hostetter, J. 2015. Gamma-delta T cell responses in subclinical and clinical stages of Bovine Mycobacterium Avium Paratuberculosis infection. Meeting Abstract. page 25.

Interpretive Summary:

Technical Abstract: The early immune response to Mycobacterium avium subsp. paratuberculosis (MAP) in cattle is characterized by a Th1-like immune response effective in controlling bacterial proliferation during the subclinical stage of infection. In young calves nearly 60% of circulating lymphocytes are gamma delta T cells. We hypothesize that gamma delta T cells promote protective immune responses in the early stages of MAP infection in cattle. Bovine gamma delta T cells are divided into subsets based upon their expression of Workshop Cluster 1 (WC1). In this study we evaluated WC1 gamma delta T cells in peripheral blood and in the ileal mucosa of cattle naturally infected with MAP. Cattle were in the subclinical or clinical stages of disease. Our data demonstrate that antigen specific proliferation of peripheral blood WC1+ gamma delt T cells was detected in sub-clinically, but not clinically infected cattle. The proliferating gamma delta T cells were composed of both WC1.1+ and WC1.2+ subtypes. When we used immunofluorescent staining on sections of ileum we did not observe significant difference in WC1+ gamma delta T cell subset distribution among control cattle or cattle in the sub-clinical or clinical stages of MAP infection. In all animals WC1.2+ gamma delta T cells were the predominate WC1+ subset in the lamina propria of the ileum. These data provide insight into the responses and distribution of WC1+ gamma delta T cells in peripheral blood and intestinal mucosa in subclinical and clinical stages of MAP infection in cattle.