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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Publications at this Location » Publication #322811

Research Project: CEREAL RUST FUNGI: GENETICS, POPULATION BIOLOGY, AND HOST-PATHOGEN INTERACTIONS

Location: Cereal Disease Lab

Title: The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus

Author
item MAGO, ROHIT - Commonwealth Scientific And Industrial Research Organisation (CSIRO)
item ZHANG, PENG - Plant Breeding Institute
item VAUTRIN, SONIA - Institut National De La Recherche Agronomique (INRA)
item SIMKOVA, HANA - Institute Of Experimental Botany
item BANSAL, URMIL - Plant Breeding Institute
item LUO, MING-CHENG - University Of California
item Rouse, Matthew - Matt
item KARAOGLU, HAYDAR - Plant Breeding Institute
item PERIYANNAN, SAMBASIVAM - Commonwealth Scientific And Industrial Research Organisation (CSIRO)
item Kolmer, James - Jim
item Jin, Yue
item AYLIFFE, MICHAEL - Commonwealth Scientific And Industrial Research Organisation (CSIRO)
item BARIANA, HARBANS - Plant Breeding Institute
item PARK, ROBERT - Plant Breeding Institute
item MCINTOSH, ROBERT - Plant Breeding Institute
item DOLEZEL, JAROSLAV - Institute Of Experimental Botany
item BERGES, HELENE - Institut National De La Recherche Agronomique (INRA)
item SPIELMEYER, WOLFGANG - Commonwealth Scientific And Industrial Research Organisation (CSIRO)
item LAGUDAH, EVANS - Commonwealth Scientific And Industrial Research Organisation (CSIRO)
item ELLIS, JEFF - Commonwealth Scientific And Industrial Research Organisation (CSIRO)
item DODDS, PETER - Commonwealth Scientific And Industrial Research Organisation (CSIRO)

Submitted to: Nature Plants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/27/2015
Publication Date: 11/30/2015
Citation: Mago, R., Zhang, P., Vautrin, S., Simkova, H., Bansal, U., Luo, M., Rouse, M.N., Karaoglu, H., Periyannan, S., Kolmer, J.A., Jin, Y., Ayliffe, M., Bariana, H., Park, R., Mcintosh, R., Dolezel, J., Berges, H., Spielmeyer, W., Lagudah, E., Ellis, J., Dodds, P. 2015. The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus. Nature Plants. 15186:1-16. doi:10.1038/nplants.2015.186.

Interpretive Summary: Wheat stem rust caused by the fungal pathogen Puccinia graminis f. sp. tritici is a major threat to wheat production worldwide. The appearance and subsequent spread of Ug99 and other virulent races have elucidated the continuing need for new resistance sources to protect against this devastating pathogen. We identify the wheat stem rust resistance gene Sr50 by physical mapping, mutation and complementation as homologous to barley Mla encoding a Coiled-Coil-Nucleotide-Binding-Leucine-Rich Repeat (CC-NB-LRR) protein. We show that Sr50 confers a unique resistance specificity, different from Sr31 and other genes on rye chromosome 1RS, and is effective against the broadly virulent Ug99 race lineage. Extensive haplotype diversity at the rye Sr50 locus holds promise for mining effective resistance genes. Sr50 is effective against a broad range of worldwide stem rust pathogen races and is the first stem rust resistance gene cloned from the tertiary gene pool of wheat. This advance will allow introgression of Sr50 into new wheat varieties separately from deleterious rye sequences and pyramiding of this gene with other broadly effective Sr genes either through conventional breeding or by transgenic delivery to provide durable stem rust control.

Technical Abstract: We identify the wheat stem rust resistance gene Sr50 by physical mapping, mutation and complementation as homologous to barley Mla encoding a Coiled-Coil-Nucleotide-Binding-Leucine-Rich Repeat (CC-NB-LRR) protein. We show that Sr50 confers a unique resistance specificity, different from Sr31 and other genes on rye chromosome 1RS, and is effective against the broadly virulent Ug99 race lineage. Extensive haplotype diversity at the rye Sr50 locus holds promise for mining effective resistance genes.