Submitted to: Talanta
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/26/2016
Publication Date: 4/30/2016
Citation: Elkin, K.R., Slingsby, R., Bryant, R.B. 2016. Determination of phytate in high molecular weight, charged organic matrices by two-dimensional size exclusion-ion chromatography. Talanta. 156-157:6-10. doi: 10.1016/j.talanta.2016.04.059. Interpretive Summary: With growing concerns regarding the mitigation of phosphorus losses in agriculture, strong interest exists in developing a rapid, robust and sensitive method for determining phosphorus losses in agricultural runoff. In cooperation with Thermo-Fisher Dionex, USDA-ARS scientists sought to develop a method to prepare and analyze samples from a majority of agricultural sources in a single, in-line method. This research discusses the utility of using such a method on a variety of matrices (water, manure and soil extracts), adding to it usefulness.
Technical Abstract: A two-dimensional chromatography method for analyzing anionic targets (specifically phytate) in complex matrices is described. Prior to quantification by anion exchange chromatography, the sample matrix was prepared by size exclusion chromatography, which removed the majority of matrix complexities. Retention of phytate on the AS11-HC was sensitive and reproducible allowing this method to provide baseline separation of phytate from a manure extract within 14 minutes. The method is simple, sensitive, reproducible (between-run variation 5% RSD) and very suitable for routine determination of ionic targets (like phytate) in complex matrices such as manure extracts.