|LILLEHOJ HYUN S|
|RUFF MICHAEL D|
Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/1994
Publication Date: N/A
Technical Abstract: The effects of tumor necrosis factor (TNF) on coccidiosis were investigated. Chicken TNF(CTNF) was measured using a new in vitro assay system. Peripheral blood lymphocyte-derived monocytes produced a TNF-like factor when stimulated with LPS or E. tenella sporozoites. CTNF was more cytotoxic to the chicken cell line, OU-2, than the mouse TNF-sensitive cell line, LM, when compared with recombinant human TNF (rhTNF). When SC and TK chickens were treated with cTNF, rhTNF, normal rabbit serum (NRS) or rabbit anti-rhTNF serum (R.anti-rhTNF) followed by E. tenella infection, cTNF depressed body weight gain (BWG) in both SC and TK chickens whereas rhTNF depressed BWG of SC but not TK chickens. R.anti-rhTNF injection partially prevented E. tenella- induced weight loss in SC chickens but not in TK chickens. TNF treatment had no effect on lesion scores. These results suggest that TNF is involved in the pathogenesis of coccidiosis.