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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Chemistry Research » Research » Publications at this Location » Publication #322254

Research Project: Disease Defense Responses Signaling in Maize

Location: Chemistry Research

Title: Over 10,000 new maize mutants added to the uniformMu public resource: now 67,000 total Mu insertions with 42% genome coverage

Author
item SAUNDERS, JONATHAN - University Of Florida
item Hunter, Charles
item WU, SHAN - University Of Florida
item SUZUKI, MASA - University Of Florida
item ANGELICA SANCLEMENTE, MARIA - University Of Florida
item LIU, PENG - University Of Florida
item AVIGNE, WAYNE - University Of Florida
item ANDORF, CARSON - University Of Florida
item SACHS, MARTY - University Of Florida
item MCCARTY, DON - University Of Florida
item KOCH, KAREN - University Of Florida

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/30/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Over 10,000 new mutants have been added to the UniformMu reverse genetics resource in release 7, bringing the total to over 67,000 germinal transposon insertions. These are available in 11,140 independent seed stocks. Close to half of the maize filtered gene set (42%) is represented by at least one Mutator insertion allele, and 65% of these genes have two or more mutant alleles. UniformMu insertion lines can be searched by gene sequence or by GRMZM maize-gene identifiers, and can also be browsed at MaizeGDB.org. Seeds can be requested free of charge through this site or directly from the Maize Genetics Cooperation Stock Center. The UniformMu population was created by introgressing a Mutator transposase into a W22 inbred background to provide a uniform phenotypic background for mutant comparisons. Each UniformMu line carries an average of 10 unique, germinal insertions. These lines are provided as segregating F3 material in a “Mu-off”, stable state with a mutable bronze1 allele that serves as a visual marker for activity of the MuDR transposase. Methods and tips for users are available at MaizeGDB.org, including information on how to request seed stocks and effectively test genotype-phenotype cosegregation.