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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Bioenergy Research » Research » Publications at this Location » Publication #322239

Research Project: Biochemical Technologies to Enable the Commercial Production of Biofuels from Lignocellulosic Biomass

Location: Bioenergy Research

Title: Process strategies to maximize lipid accumulations of novel yeast in acid and base treated hydrolyzates

item Slininger, Patricia - Pat
item Dien, Bruce
item Moser, Bryan
item Bakota, Erica
item Evangelista, Roque
item Thompson, Stephanie
item O Bryan, Patricia
item Cotta, Michael
item BALAN, VENKATESH - Michigan State University
item XUE, YONGKANG - Michigan State University
item JIN, MINGJIE - Michigan State University
item ORJUELA, ANDREA - Michigan State University
item DE COSTA SOUSA, LEONARDO - Michigan State University
item DALE, BRUCE - Michigan State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/27/2015
Publication Date: 4/28/2015
Citation: Slininger, P.J., Dien, B.S., Moser, B.R., Bakota, E.L., Evangelista, R.L., Thompson, S.R., O Bryan, P.J., Cotta, M.A., Balan, V., Xue, Y., Jin, M., Orjuela, A., De Costa Sousa, L., Dale, B. 2015. Process strategies to maximize lipid accumulations of novel yeast in acid and base treated hydrolyzates [abstract].

Interpretive Summary:

Technical Abstract: Oleaginous yeasts can accumulate up to 70% of cell biomass as lipids, predominantly as triacylglycerols. Yeast lipid fatty acid profiles have been reported to be similar to that of vegetable oils and consist primarily of oleic, palmitic, stearic, and linoleic acids. This capability provides the opportunity to use yeasts to produce bio-based fuels and chemicals from agricultural residues and bioenergy crops, such as corn stover and switchgrass, respectively. Successful cultivation of these yeasts on inhibitory lignocellulosic hydrolyzates is uncertain because little has been published regarding their application to bioconversion of plant biomass. Novel yeasts producing promising lipid concentrations (>10 g/L) from enzyme hydrolyzates of acid and base pre-treated lignocellulosic biomass were discovered in a prior screening of the ARS Culture Collection (NCAUR, Peoria, IL). Process strategies for maximizing lipid production in one of the more benign hydrolyzates, AFEX-pretreated corn stover, and one of the more toxic hydrolyzates, dilute acid-pretreated switchgrass, respectively, were employed. Hydrolyzates were prepared at up to 20% solids loading to provide over 100 g/L sugars (at ~60:40 mass ratio glucose:xylose). Variables examined included hydrolyzate concentration, nitrogen source, C:N ratio, pH, temperature, and process train design. Depending upon plant biomass yields, we estimated that oleaginous yeasts have the capability of producing ~48 and 190 gal oil per acre from corn stover and switchgrass, respectively. As a frame of reference, ~68 gallons of oil per acre are produced from processing soybeans. Results are discussed with respect to advances made and limitations still to be addressed in future work.