|YANG, CHUANYU - University Of Florida|
|ZHANG, MUQING - University Of Florida|
|Shatters, Robert - Bob|
|POWELL, CHARLES - University Of Florida|
Submitted to: International Research Conference on Huanglongbing
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/2014
Publication Date: 3/30/2015
Citation: Yang, C., Zhang, M., Duan, Y., Shatters, R.G., Powell, C. 2015. Optimization of an efficient transcuticular delivery system for control of citrus huanglongbing. J Cit Pathol. 2(1):47. iocv_journalcitruspathology_30222.
Technical Abstract: We experimentally develop and optimize a transcuticular nano-delivery system for enhancing permeation of effective compound against HLB disease through citrus cuticle into the phloem by foliar spray or bark application. The results showed that two kinds of nanoemulsions (W/O and O/W) with the smallest drop size of 13.68±0.26nm were prepared for loading the antimicrobial compounds. The results also indicated that cuticles were more difficult to be isolated from valencia orange (Citrus sinensis) and HLB-symptomatic leaves. Brij 35 was screened from a pool of eight candidate penetrants as an optimal penetrant for HLB-affected cuticle, exhibiting an approximately 3-fold enhancement of cuticular permeability over water control. In vitro assay, the nano formulations made with Amp which was loaded into nano-emulsions and coupled with Brij 35 resulted in higher inhibitory zone diameters (5.75mm and 6.66mm), compared with inhibitory zone diameters of Amp coupled with nanoemulsions (3.02mm and 4.90mm), or Brij35 (4.34mm) and Amp solution along (2.83mm). Furthermore, the formulations more efficiently eliminated the Candidatus Liberibacter asiaticus in HLB-affected citrus in planta.