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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Publications at this Location » Publication #321017

Research Project: EMERGING DISEASES OF CITRUS, VEGETABLES, AND ORNAMENTALS

Location: Subtropical Plant Pathology Research

Title: FLS2 from Solanum tuberosum have interaction with FlaLas from Candidatus Liberibacter asiaticus

Author
item Cui, Yiping
item Liu, Guodong
item Powell, Charles
item Duan, Ping

Submitted to: International Research Conference on Huanglongbing
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/2014
Publication Date: 3/30/2015
Citation: Cui, Y., Liu, G., Powell, C., Duan, Y. 2015. FLS2 from Solanum tuberosum have interaction with FlaLas from Candidatus Liberibacter asiaticus. J Cit Pathol. 2(1):9. iocv_journalcitruspathology_30222.

Interpretive Summary:

Technical Abstract: Candidatus Liberibacter asiaticus’ (Las) is a Gram-negative and phloem-limited alphaproteobacterium. Las attacks all citrus species and citrus hybrids in the genus of Citrus and other relatives, and causes a systemic disease. Currently, control of this devastating disease is extremely difficult since no highly resistant cultivars are available. Therefore, the cloning of resistance genes from alternative plants may be a viable option. As indicated in a previous report, Las encodes a functional flagellin (Fla), which contains a conserved 22 amino acid domain (flg22Las). In this study, 108 potato genotypes were screened for an increase in reactive oxygen species (ROS) activity induced by the flg22Las. The potato genotype TC72 and TC104 showed high ROS activity in addition to an increase in the expression level of resistance-related genes, especially the Flagellin-sensing 2 gene (StFLS2), post Agrobacterium-mediated transient expression of flaLas. It is worth noting there are two different FLS2 sequences in the published potato genome, StFLS2a and StFLS2b, which differ in the number of Leucine-rich repeats and in amino acid sequence. Our results demonstrate that only StFLS2a was up-regulated and not both copies of StFLS2 when challenged with FlaLas in the selected potato plants. Yeast-two-hybrid (Y2H) assays and Bimolecular fluorescence complementation (BiFc) approaches were employed to further elucidate the interaction between FlaLas and StFLS2a. Results showed that the interaction between FlaLas and StFLS2a localized to the plant cell membrane. In an effort to obtain HLB resistant citrus, we are generating transgenic citrus expressing StFLS2a.