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ARS Home » Southeast Area » Byron, Georgia » Fruit and Tree Nut Research » Research » Publications at this Location » Publication #320694

Title: Resistance evaluation of Pera (Citrus sinensis) genotypes to citrus canker in greenhouse conditions

item GONCALVES-ZULIANI, ALINE - University Of Maringa
item NOCCHI, PAULA - University Of Maringa
item FRIAS, A. - University Of Maringa
item NANAMI, D. - University Of Maringa
item SOARES, L. - University Of Maringa
item SOUZA, H. - University Of Maringa
item Bock, Clive
item NUNES, WILLIAM - University Of Maringa

Submitted to: American Phytopathology Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/8/2015
Publication Date: 11/1/2015
Citation: Goncalves-Zuliani, A.M., Nocchi, P.T., Frias, A.A., Nanami, D.S., Soares, L.S., Souza, H.S., Bock, C.H., Nunes, W.W. 2015. Resistance evaluation of Pera (Citrus sinensis) genotypes to citrus canker in greenhouse conditions. American Phytopathology Society. 105(Suppl. 4):S4.51.

Interpretive Summary: Abstract only. JLR

Technical Abstract: Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri results in serious yield losses and phytoregulation penalties. The use of resistant genotypes is recognized as an important tool to facilitate control of the pathogen. Studies have show that artificial inoculation results in typical symptoms of citrus canker (using injury, followed by spraying or infiltration of the inoculum into the plant tissue). This study evaluated the resistance of genotypes of the sweet orange variety Pera to X. citri under partially controlled conditions (in a greenhouse). A total of 25 Pera genotypes were evaluated in two trials. The leaves were inoculated by needle punching (0.55 x 0.20 mm), infiltration of inoculum and maintaining material uder constant wetness. The inoculum was adjusted to a concentration of 108 CFU/mL and evaluations were made measuring lesions diameter. Bacterial quantification was performed by counting the Colony Forming Unit (CFU) isolated from each lesion. The results showed that the genotypes EEL Pera and IAC2000/1 had the smallest lesion diameters both assays, moreover, Bianchi/CC Pera and IAC obtained the smallest diameters in the second assay and the lowest bacterial populations, suggesting that these genotypes have a greater level of resistance to the pathogen.