Location: Fruit and Tree Nut ResearchTitle: Development of microsatellite markers for Fusicladium effusum, the causal agent of pecan scab
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/8/2015
Publication Date: 11/1/2015
Citation: Bock, C.H., Chen, C., Stevenson, K.L., Wood, B.W. 2015. Development of microsatellite markers for Fusicladium effusum, the causal agent of pecan scab. Phytopathology. 105(Suppl. 4):S4.17.
Interpretive Summary: Abstract only.
Technical Abstract: Pecan scab (caused by F. effusum) is the most important diseases of pecan in the southeastern U.S. Microsatellite (simple sequence repeat, SSR) motifs were mined from the genome of Fusicladium effusum assembled from 454 pyrosequencing and Illumina Miseq reads. A total of 278 SSR primers were designed. The lengths of the predicted amplicons ranged from 119 to 209 bp, and contained 2 to 6 repeat units of di- to septo-nucleotide sequences. The primers were used to screen 48 F. effusum isolates sampled from 11 populations of the pathogen throughout the southeastern U.S., using a 3500 Genetic Analyzer. Only 33 of the SSR primers were found to be both reliable and polymorphic. The number of polymorphic alleles among these codominant markers ranged from 2 to 19, and the 33 primers differentiated all the 48 isolates from the 11 populations. Polymorphic loci ranged from 69.7-97.0% depending on the populations of F. effusum that were screened. These SSR primers should be useful in studying the genetic diversity and population structure of the pathogen.