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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #319021
Title: A SNP genetic linkage map based on the ‘Hamilton’ by ‘Spencer’ recombinant inbred line (RIL) population identified QTL for seed Isoflavone contents in soybean

Author
item AKOND, MASUM - Fayetteville State University
item LUI, SHIMING - Southern Illinois University
item KANTARTZI, STELLA - Southern Illinois University
item MEKSEM, KHALID - Southern Illinois University
item Bellaloui, Nacer
item LIGHTFOOT, DAVID - Southern Illinois University
item YUAN, JIAZHENG - Michigan State University
item WANG, DECHUN - Michigan State University
item ANDERSON, JAMES - Southern Illinois University
item KASSEM, ABDELMAJID - Fayetteville State University

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/24/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary: Soybean seed quality is determined by its composition including protein, oil, fatty acids, and sugars. Soybean seed also contains phytohormones (plant hormones) called isoflavones, and they are thought to have beneficial effects on human health. Therefore, increasing this compound and controlling its production genetically is crucial. DNA marker (indicator) to identify isoflavones content in seed was used as an approach in the current experiment. Two new chromosomal (DNA) regions were identified and related to isoflavones in seeds in soybean population. One major region controlled both daidzein and total isoflavone contents was found on Chromosome 6. A second region associated with glycitein content was identified on the chromosome 18. These two new regions could be used in soybean breeding to optimize isoflavone content.

Technical Abstract: Soybean is one of the most important crops worldwide for its protein, oil as well as the health beneficial phytoestrogens or isoflavone. This study reports a relatively dense SNP-Based genetic map based on ‘Hamilton’ by ‘Spencer’ recombinant inbred line (RIL) population and quantitative trait loci (QTL) for seed isoflavone contents. The genetic map is composed of 1,502 SNP markers and covers about 1,423.72 cM of the soybean genome. Two QTL for seed isoflavone contents have been identified in this population. One major QTL controlled both daidzein (qDZ1) and total isoflavone contents (qTI1) was found on LG C2 (Chr 6). A second QTL for glycitein content (qGT1) was identified on the LG G (Chr 18). These two QTL in addition to others identified in soybean could be used in soybean breeding to optimize isoflavone content. This newly assembled soybean linkage map is a useful tool to identify and map QTL for important agronomic traits and enhance the identification of the genes involved in these traits.