Skip to main content
ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #318861
Title: Transcriptome analysis to identify differentially expressed genes associated with ty-5 against tomato yellow leaf curl virus and Sw-7 against tomato spotted wilt virus in tomato

Author
item Padmanabhan, Chellappan
item ZHENG, YI - Boyce Thompson Institute
item SHEKASTE-BAND, REZA - University Of Florida
item SCOTT, JOHN - University Of Florida
item FEI, ZHANGJUN - Boyce Thompson Institute
item Ling, Kai-Shu

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/16/2015
Publication Date: 9/14/2015
Citation: Padmanabhan, C., Zheng, Y., Shekaste-Band, R., Scott, J.W., Fei, Z., Ling, K. 2015. Transcriptome analysis to identify differentially expressed genes associated with ty-5 against tomato yellow leaf curl virus and Sw-7 against tomato spotted wilt virus in tomato. 3rd Plant Genomics Congress=USA. Meeting Abstract. P.2.

Interpretive Summary: N/A

Technical Abstract: Tomato yellow leaf curl virus (TYLCV) and Tomato spotted wilt virus (TSWV) are two of the most economically important viruses on tomato (Solanum lycopersicum) worldwide. Developing a cultivar with resistance would be the most effective and economical means for viral disease management. However, identification and characterization of a resistant gene derived from a wild species is not an easy task. Translational genomic analysis might facilitate the process in identifying the potential resistance genes. In the present study, transcriptome analysis with RNA-seq was used to uncover differentially expressed (DE) genes from tomato near isogenic breeding lines introgressed between the susceptible and R-lines (ty-5 and Sw-7) upon TYLCV or TSWV infection, respectively. Total RNA preparations were extracted using leaf tissues collected from plants at 4, 7, 14, 21, 35 days post inoculation. RNA-seq was conducted and transcriptome analysis was performed to assess gene expression between the resistant and susceptible tomato plants at each time period. Results showed TYLCV, highly expressed genes in the R-lines (ty5), were shown to involve functions in plant defense, like NBS-LRR and CC-NBS-LRR resistance proteins, proteinase inhibitor, and transcription factors. The down regulated or not expressed genes in the R-line appeared to have roles in facilitating the establishment of early and systemic infection, such as chloroplast, development, vacuolar sorting, transcriptional factor and signal transduction. In the case of TSWV, highly expressed genes in the R-lines (Sw-7) showed roles in plant defense, including genes like pathogen-induced calmodulin-binding protein, chloroplast synthesis, metabolism, kinase, PR protein, chitinase, stress protein, and proteinase inhibitor. Genes that were down regulated or not expressed in the R-line appeared to have roles in regulating systemic symptom development, including those genes in metabolism, chloroplast, proteinase inhibitor, and transcription factors. Characterization of the gene expression upon virus infection would facilitate the understanding of the mechanism of virus resistance and may allow us to identify the virus-resistance gene