|Patil, Ujwal - University Of New Orleans|
|Osorno, Laura - University Of New Orleans|
|Ellender, Angela - University Of New Orleans|
|Tarr, Matthew - University Of New Orleans|
Submitted to: Analytical Biochemistry
Publication Type: Abstract Only
Publication Acceptance Date: 5/7/2015
Publication Date: 9/1/2015
Citation: Patil, U.S., Osorno, L., Ellender, A., Grimm, C.C., Tarr, M.A. 2015. Cleavable ester linked magnetic nanoparticles for labeling of solvent exposed primary amine groups of peptides/proteins. Analytical Biochemistry. 484:18-20.
Technical Abstract: In order to study the solvent exposed lysine residues of peptides/proteins, we previously reported disulfide linked N-hydrosuccinimide ester modified silica coated iron oxide magnetic nanoparticles (NHS-SS-SiO2@Fe3O4 MNPs). The presence of a disulfide bond in the linker limits the use of disulfide reducing agent during protein digestion and allows unwanted disulfide formation between the MNP and protein. In the current work, the disulfide bond was replaced with a cleavable ester group to synthesize NHS ester modified SiO2@Fe3O4 MNPs. Use of the cleavable ester group provides and improved method for protein labeling and allows use of disulfide reducing agents during protein digestion.