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Research Project: Development of Management Strategies to Mitigate Pre-harvest Microbial-derived Off-flavors in Fish Grown in Aquaculture

Location: Natural Products Utilization Research

Title: Antibacterial activities of metabolites from Platanus occidentalis (American sycamore) against fish pathogenic bacteria

Author
item Schrader, Kevin
item Hamann, Mark - University Of Mississippi
item Mcchesney, James - Ironstone Separations, Inc
item Rodenburg, Douglas - Ironstone Separations, Inc
item Ibrahim, Mohamed - University Of Mississippi

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 7/17/2015
Publication Date: 9/14/2015
Citation: Schrader, K., Hamann, M.T., Mcchesney, J.D., Rodenburg, D.L., Ibrahim, M.A. 2015. Antibacterial activities of metabolites from Platanus occidentalis (American sycamore) against fish pathogenic bacteria. Proceedings of World Congress and Exhibition on Antibiotics, September 14-16, 2015, Las Vegas, Nevada. 6(4):73.

Interpretive Summary:

Technical Abstract: One approach to the management of common fish diseases in aquaculture is the use of antibiotic-laden feed. However, there are public concerns about the use of antibiotics in agriculture and the potential development of antibiotic resistant bacteria. Therefore, the discovery of other environmentally safe natural compounds as alternatives to antibiotics would benefit the aquaculture industries. Four natural compounds, commonly called platanosides, [kaempferol 3-O-a-L-(2",3"-di-E-p-coumaroyl)rhamnoside (1), kaempferol 3-O-a-L-(2"-E-p-coumaroyl-3"-Z-p-coumaroyl)rhamnoside (2), kaempferol 3-O-a-L-(2"-Z-p-coumaroyl-3"-E-p-coumaroyl)rhamnoside (3), and kaempferol 3-O-a-L-(2",3"-di-Z-p-coumaroyl)rhamnoside (4)] isolated from the leaves of the American sycamore (Platanus occidentalis) tree were evaluated using a rapid bioassay for their antibacterial activities against common fish pathogenic bacteria including Flavobacterium columnare, Edwardsiella ictaluri, Aeromonas hydrophila, and Streptococcus iniae. The four isomers and a mixture of all four isomers were strongly antibacterial against isolates of F. columnare and S. iniae. Against F. columnare ALM-00-173, 3 and 4 showed the strongest antibacterial activities, with 24-h 50% inhibition concentration (IC50) values of 2.13±0.11 and 2.62±0.23 mg/L, respectively. Against S. iniae LA94-426, 4 had the strongest antibacterial activity, with 24-h IC50 of 1.87±0.23 mg/L. Neither a mixture of the isomers nor any of the individual isomers were antibacterial against isolates of E. ictaluri and A. hydrophila at the test concentrations used in the study. Several of the isomers appear promising for the potential management of columnaris disease and streptococcosis in fish.