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Title: Development of ZYMV-resistant lines using genomic tools

Author
item Levi, Amnon
item Ling, Kai-Shu

Submitted to: International Symposium on Cucurbits Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/27/2015
Publication Date: 6/22/2015
Citation: Levi, A., Ling, K. 2015. Development of ZYMV-resistant lines using genomic tools. International Symposium on Cucurbits Proceedings. 41. https://www.verticesur.es/congresos/CUCURBITS2015/programme.php

Interpretive Summary: N/A

Technical Abstract: The aphid-transmitted potyviruses of watermelon, including papaya ringspot virus (PRSV), watermelon mosaic virus (WMV), and zucchini yellow mosaic virus (ZYMV) cause serious damage to the watermelon crop throughout the world. The United States Plant Introduction (PI) 595203 is resistant to ZYMV-FL and ZYMV-CT and the resistance is conferred by a single recessive gene. In a previous study, we identified the eukaryotic elongation factor eIF4E allele of PI 595203 is linked to ZYMV-resistance. We developed two cleaved amplified polymorphic sequences (CAPS) markers within eIF4E linked (6 cM) and a sequence characterized amplified region (SCAR) marker linked (8 cM) to the ZYMV-FL resistance gene locus. The objective of this study was to incorporate the ZYMV-resistance into watermelon cultivars using phenotyping and genotyping (MAS) criteria in a backcross breeding and a recurrent selection scheme. We were able to develop BC3F6 and BC4F5 watermelon breeding lines with sufficient fruit quality and ZYMV-resistance. The eIF4e (watermelon sequence Cla019623) located on chromosome 3 of the watermelon genome (locus=Chr3:7767735:7770583; www.icugi.org) consistently co-segregated with ZYMV-resistance and might be useful for incorporating ZYMV-resistance from PI 595203 into the genomic background of watermelon cultivars.