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Title: ANALYSIS OF METALLOPROTEINS AND METAL-BINDING PEPTIDES BY CAPILLARY ELECTROPHORESIS

Author
item Richards, Mark
item BEATTIE JOHN H - THE ROWETT RESEARCH INST

Submitted to: Journal of Capillary Electrophoresis
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/18/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary: This manuscript reviews the applications of a new analytical technology called capillary electrophoresis to the separation and characterization of specific metal-binding proteins and peptides. Metalloproteins and metal-binding peptides play important catalytic and structural roles within the cell and as such are critical to normal cellular processes such as respiration, metabolism, gene expression, and metal homeostasis. Specific methods to determine structure and functionality of these proteins and peptides as well as methods to quantify the interaction between the metal and the protein or peptide are discussed. Specific examples such as transferrin, albumin, hemoglobin, metallothionein, glutathione and others are included to emphasize the usefulness of capillary electrophoresis for the analysis of these and other important molecules. The objective of this review was to demonstrate what has been done to date and to offer suggestions as to how this technique might be applied to future analyses. This information will be useful for investigators requiring a rapid and sensitive method to analyze for metalloproteins and metal-binding peptides.

Technical Abstract: This review discusses the application of capillary electrophoresis (CE) to the separation and analysis of metalloproteins and peptides. Specific examples include serum proteins, metalloenzymes, heme-containing proteins, metal-binding proteins and metal-binding peptides. Examples from each of these categories are discussed in the context of characterization and purity assessment, structural studies, metal-binding and stability studies, metalloenzyme activity determined both on- and off-line, and analyses with implications for clinical diagnoses. Also covered are references to specific modes of CE used to perform the separations as well as some specific methods of detection. The intent of this review is to discuss selected examples of metalloprotein and metal-binding peptide analyses conducted using CE which emphasize the utility of this technique as well as its potential for new and wider application.