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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #318182
Title: The mitochondrial genome of a Texas outbreak strain of the cattle tick, Rhipicephalus (Boophilus) microplus, derived from whole genome sequencing Pacific Biosciences and Illumina reads

Author
item MCCOOKE, JOHN - Murdoch University
item Guerrero, Felicito
item BARRERO, ROBERTO - Murdoch University
item BLACK, MICHAEL - Murdoch University
item HUNTER, ADAM - Murdoch University
item BELL, CALLUM - National Center For Genome Resources
item FAYE, SCHILKEY - National Center For Genome Resources
item Miller, Robert
item BELLGARD, MATTHEW - Murdoch University

Submitted to: Elsevier
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/22/2015
Publication Date: 8/10/2015
Citation: Mccooke, J., Guerrero, F., Barrero, R., Black, M., Hunter, A., Bell, C., Faye, S., Miller, R., Bellgard, M. 2015. The mitochondrial genome of a Texas outbreak strain of the cattle tick, Rhipicephalus (Boophilus) microplus, derived from whole genome sequencing Pacific Biosciences and Illumina reads. Elsevier. Gene. 571(1):135-141.

Interpretive Summary: The cattle fever tick, Rhipicephalus (Boophilus) microplus is one of the most significant medical veterinary pests in the world, as it is able to transmit microbes that cause several serious livestock diseases. This tick negatively impacts agricultural economies of tropical and subtropical countries around the world. Mitochondria are organelles that play important roles in cellular functioning. In our study, we sequenced the complete R. microplus mitochondrial genome from the Deutch strain from Texas, USA. We compared the Deutch strain mitochondrion genome to that from a Brazilian R. microplus and from an Australian cattle tick that has recently been taxonomically re-designated as Rhipicephalus australis after previously being considered R. microplus. The sequence differences of the Texas and Australia ticks are much higher than the differences seen in comparing the Texas and Brazil ticks, confirming evidence that the Australian cattle ticks are a distinct species from the R. microplus of the Americas.

Technical Abstract: The cattle fever tick, Rhipicephalus (Boophilus) microplus is one of the most significant medical veterinary pests in the world, vectoring several serious livestock diseases negatively impacting agricultural economies of tropical and subtropical countries around the world. In our study, we assembled the complete R. microplus mitochondrial genome from Illumina and Pac Bio sequencing reads obtained from the ongoing R. microplus (Deutch strain from Texas, USA) genome sequencing project. We compared the Deutch strain mitogenome to the mitogenome from a Brazilian R. microplus and from an Australian cattle tick that has recently been taxonomically designated as Rhipicephalus australis after previously being considered R. microplus. The sequence divergence of the Texas and Australia ticks is much higher than the divergence between the Texas and Brazil ticks, confirming evidence that the Australian ticks are distinct from the R. microplus of the Americas.