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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #317968
Title: Effects of three novel resistant black raspberry selections on Amphorophora agathonica feeding behavior and performance

Author
item LIGHTLE, DANIELLE - University Of California Agriculture And Natural Resources (UCANR)
item DOSSETT, MICHAEL - Agriculture And Agri-Food Canada
item EBERT, TIM - University Of Florida
item Finn, Chad
item Martin, Robert
item Lee, Jana

Submitted to: Arthropod-Plant Interactions
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/2/2015
Publication Date: 8/5/2015
Citation: Lightle, D., Dossett, M., Ebert, T., Finn, C.E., Martin, R.R., Lee, J.C. 2015. Effects of three novel resistant black raspberry selections on Amphorophora agathonica feeding behavior and performance. Arthropod-Plant Interactions. 9:487-496. doi: 10.1007/s11829-015-9390-z.

Interpretive Summary: Using aphid-resistant raspberries is a practical and cost-effective approach for growers to manage aphid vectors and virus infection. Recently, three new black raspberry selections (ORUS 3778-1, ORUS 3817-1, and ORUS 4109-1) were identified as resistant to the large raspberry aphid. We studied stages of host plant acceptance: whether aphids were attracted to these host plants, their rate of producing nymphs while feeding on these plants, survival of nymphs, and adult feeding behavior (using the electrical penetration graph (EPG)) to identify the location of the plant resistance. Aphids were more attracted to the susceptible cultivar Munger (control) than to ORUS 3778-1 and ORUS 3817-1, while equally attracted to the control and ORUS 4109-1. Adults still deposited nymphs on the resistant selections, but deposited fewer nymphs on resistant lines relative to the susceptible control. Nymphs survived only an average of 3.3-3.6 days on resistant selections. There were differences in feeding behavior between the susceptible control and the resistant selections, but no differences between the three resistant selections. The tissue responsible for resistance appears to be the phloem sieve elements. Because feeding behavior of A. agathonica did not differ between the three resistant selections, independent confirmation that resistance is conferred by unique genes should be obtained before pyramiding these sources together.

Technical Abstract: Host plant resistance is a practical and cost-effective approach for growers to manage insect pests. Recently, three new sources of resistance in black raspberry (selections ORUS 3778-1, ORUS 3817-1, and ORUS 4109-1) against the large raspberry aphid, Amphorophora agathonica, were identified. We studied stages of host plant acceptance: host plant attraction, parturition (deposition of nymphs), nymph survival, and adult feeding behavior (using the electrical penetration graph (EPG)) to identify the location of the plant resistance. Aphids were more attracted to the susceptible cultivar Munger (control) than to ORUS 3778-1 and ORUS 3817-1, while equally attracted to the control and ORUS 4109-1. Parturition occurred on the resistant selections, but fewer nymphs were deposited on resistant lines relative to the susceptible control. Nymphs survived only an average of 3.3-3.6 days on resistant selections. There were differences in feeding behavior between the susceptible control and the resistant selections, but no differences between the three resistant selections. The tissue responsible for resistance appears to be the phloem sieve elements. Aphids had a reduced probability of salivation into the phloem sieve elements of resistant selections, and only one aphid each on ORUS 3778-1 and ORUS 4109-1 successfully ingested from the phloem. Because feeding behavior of A. agathonica did not differ between resistant selections, independent confirmation that resistance is conferred by unique genes should be obtained before pyramiding these sources together.