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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #317587

Research Project: Understanding Genetic and Physiological Factors Affecting Nutrient Use Efficiency of Dairy Cattle

Location: Animal Genomics and Improvement Laboratory

Title: Haplotypes in the promoter region of the CIDEC gene associated with growth traits in Nanyang cattle

Author
item WANG, JING - Northwest Agricultural & Forestry University
item HUA, LIU-SHUAI - Henan Agricultural University
item PAN, HONG - Northwest Agricultural & Forestry University
item ZHANG, LIANG-ZHI - Northwest Agricultural & Forestry University
item LI, MING-XUN - Northwest Agricultural & Forestry University
item HUANG, YONG-ZHEN - Northwest Agricultural & Forestry University
item LI, ZHUAN-JIAN - Northwest Agricultural & Forestry University
item LAN, XIAN-YONG - Northwest Agricultural & Forestry University
item LEI, CHU-ZHAO - Northwest Agricultural & Forestry University
item Li, Congjun - Cj
item CHEN, HONG - Northwest Agricultural & Forestry University

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/15/2015
Publication Date: 6/20/2015
Citation: Wang, J., Hua, L., Pan, H., Zhang, L., Li, M., Huang, Y., Li, Z., Lan, X., Lei, C., Li, C., Chen, H. 2015. Haplotypes in the promoter region of the CIDEC gene associated with growth traits in Nanyang cattle. Scientific Reports. 5, 12075(2015).

Interpretive Summary: Cell death-inducing DFFA-like effector c (CIDEC, also known as Fsp27) has emerged as an important regulator of metabolism. The mechanism between this gene and livestock growth traits, however, has yet to be reported. In this study, ten novel single-nucleotide polymorphism (SNP)s were found in gene regulation region of 22 transcription factors. These combinations of SNPs and gene regulation regions may have affected transcription activity of CIDEC, thus affecting the growth traits of cattle.

Technical Abstract: Cell death-inducing DFFA-like effector c (CIDEC, also known as Fsp27) has emerged as an important regulator of metabolism associated with lipodystrophy, diabetes, and hepatic steatosis. It is required for unilocular lipid droplet formation and optimal energy storage. The mechanism between this gene and livestock growth traits, however, has yet to be reported. In this study, we found ten novel SNPs in the 5’ transcriptional region of CIDEC in Nanyang (NY) cattle. These SNPs are located in the recognition sequences (potential cis-acting elements) of 22 transcription factors, and the nine haplotypes represent nine combinations of polymorphic potential cis-acting elements. The results indicated that individuals with the H8-H8 diplotype had heavier body weights and faster growth rates (P < 0.01) at 18 months than those with H1-H8. To verify the association results, we evaluated the transcriptional activities of different haplotypes in vitro, and the results were consistent with the association analysis. The H8 haplotype had 1.88-fold (P < 0.001) higher transcriptional activity than the H1 haplotype. We speculate that the haplotypes of the potential cis-acting elements may have affected the transcriptional activity of CIDEC, thus affecting the growth traits of cattle. This information may be used in molecular marker-assisted selective cattle breeding in the future.