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ARS Home » Southeast Area » Stoneville, Mississippi » Genomics and Bioinformatics Research » Research » Publications at this Location » Publication #317334

Research Project: Genomics and Bioinformatics Research in Agriculturally Important Organisms

Location: Genomics and Bioinformatics Research

Title: Sesbania bispinosa, a new host of a begomovirus-betasatellite complex in Pakistan

Author
item ZAIDI, SYED - National Institute Of Biotechnology And Genetic Engineering (NIBGE)
item AMIN, IMRAN - National Institute Of Biotechnology And Genetic Engineering (NIBGE)
item IQBAL, ZAFAR - National Institute Of Biotechnology And Genetic Engineering (NIBGE)
item AKHTAR, KHALID - National Institute Of Agricultural Botany (NIAB)
item Scheffler, Brian
item MANSOOR, SHAHID - National Institute Of Biotechnology And Genetic Engineering (NIBGE)

Submitted to: Canadian Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/2/2015
Publication Date: 1/1/2016
Citation: Zaidi, S.S., Amin, I., Iqbal, Z., Akhtar, K.P., Scheffler, B.E., Mansoor, S. 2016. Sesbania bispinosa, a new host of a begomovirus-betasatellite complex in Pakistan. Canadian Journal of Plant Pathology. 38:1, 107-111, DOI:10.1080/07060661.2015.1128980.

Interpretive Summary: Cotton leaf curl virus (CLCuV) is a devastating virus that greatly reduces cotton yields in Pakistan and India. The disease is considered a top 20 threat to US agriculture. In this study, plants of Sesbania bispinosa were found in a CLCuV infected cotton field within Pakistan. These plants were showing symptoms similar to cotton plants infected with CLCuV. Expansion of the host range of CLCuV is of great concern. DNA samples were collected from the infected plants and were examined for the presence of viruses. In this instance, the virus was related to Pedilanthus leaf curl virus and no CLCuV virus was detected. A Pedilanthus leaf curl virus has been detected in soybeans but never reported in Sesbania bispinosa which was a novel finding and thus was worthy of reporting.

Technical Abstract: Severe leaf curling, yellowing and vein thickening symptoms, typical of begomoviruses infection, were observed on Sesbania bispinosa grown in cotton leaf curl disease affected cotton field in Pakistan. A begomovirus and its associated betasatellite were amplified and sequenced from these plants. Complete nucleotide sequence shows that a monopartite begomovirus named Pedilanthus leaf curl virus (PedLCV) is associated with the disease and it shares highest sequence identity with a clone previously reported from soybean. Characterization of the betasatellite shows that it has highest level of sequence identity with Tobacco leaf curl betasatellite (TbLCB), previously isolated from soybean. Our data shows the expansion in the host range of begomovirus- betasatellite complexes. To the best of our knowledge, this report is the first on the occurrence of PedLCV and TbLCV on S. bispinosa anywhere in the world.