Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 6/26/2015
Publication Date: 11/1/2015
Citation: Hinton Jr, A. 2015. Growth of Campylobacter Incubated Aerobically in Media Supplemented with Peptones [abstract]. Campylobacter Helicobacter and Related Organisms International Workshop. p.124.
Technical Abstract: Growth of Campylobacter cultures incubated aerobically in media supplemented with peptones was studied, and additional experiments were conducted to compare growth of the bacteria in media supplemented with peptones to growth in media supplemented with fumarate-pyruvate-minerals-vitamins (FPMV). A basal medium composed of (g/l) tryptose, 10; yeast extract, 5; agar, 1.5; and NaHCO3, 0.05 was prepared, then supplemented with 5% (w/v) peptones (beef extract, lactalbumin hydrolysate, or soytone). Campylobacter growth in peptone media was determined by inoculating media with 104 cfu/ml of Campylobacter coli, Campylobacter fetus, Campylobacter jejuni, or Campylobacter lari and measuring culture optical density (OD) during aerobic incubation for 48 h at 37C. Additional experiments compared cfu/ml of Campylobacter recovered from inoculated peptone or FPMV media incubated aerobically for 48 h at 37C in culture flasks. After aerobic incubation, cfu/ml were enumerated on selective Campylobacter agar incubated microaerophilically for 48 h at 37C. Results indicated significant (p < 0.05) increases in OD of all isolates, except C. jejuni 33560, after 48 h of aerobic incubation in media supplemented with beef extract or soytone. Additionally, there was a 4-5 log cfu/ml increase in all isolates cultured aerobically for 48 h in media supplemented with beef extract or soytone, and a 4-5 log increase in C. fetus, C. coli, and C. jejuni 33560 cultured in media supplemented with FPMV. No Campylobacter were recovered from media that was not supplemented with peptones or FPMV. Findings indicate that beef extract and soytone contain metabolites that support growth of Campylobacter incubated aerobically. These media might provide a less expensive, simplified alternative for culturing Campylobacter.