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ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #316972

Title: Influence of root exudates on attachment of Pasteuria penetrans to Meloidogyne arenaria

item CHANG, LIU - University Of Georgia
item Timper, Patricia - Patty
item MEKETE, T - University Of Florida
item JOSEPH, S - University Of Florida

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 7/20/2015
Publication Date: 12/5/2015
Citation: Chang, L., Timper, P., Mekete, T., Joseph, S. 2015. Influence of root exudates on attachment of Pasteuria penetrans to Meloidogyne arenaria. Journal of Nematology. 47:252-253.

Interpretive Summary:

Technical Abstract: We hypothesized that root exudates would influence the spore attachment of Pasteuria penetrans to root-knot nematodes (Meloidogyne arenaria). An experiment was carried out using a factorial arrangement of two single spore (SS) lines cultured from P. penetrans and three single egg mass(SEM)lines cultured from M. arenaria to test the influence of root exudates on spore attachment to second-stage juveniles (J2). The root exudates were obtained by placing three 1-month-old eggplant seedlings in a foil-covered 100ml beaker containing 60 ml of water to submerge the roots and incubating in a greenhouse for 24 h. The J2 from each SEM line were incubated in root exudates for 6 h before 105 spores of P. penetrans were added to the solution and incubated for an additional for 6 h. The control included J2 incubated in sterilized water instead of root exudates. The experiment was conducted twice (Trial 1 and Trial 2). Compared to the control, exposure to root exudates reduced (P<0.0001) attachment of both SS lines to SEM14 and reduced (P=0.018) attachment of one SS line to SEM3 in Trial 1. Attachment of SS16 to SEM14 was reduced by 76% and attachment of SS25 to SEM3 and SEM14 was reduced by 54% and 85%, respectively. In Trial 2, exposure to root exudates reduced (P<0.0001) spore attachment in all three SEM lines to both SS lines compared to the control. Spore attachment was reduced by 71%, and 69% for SS16 and SS25, respectively. Differences between the two trials may be due to the method of J2 harvest. In Trial 1, J2 were obtained from roots incubated in a mist chamber where they were pre-exposed to root exudates. However, in Trial 2, the J2 were obtained from eggs that were extracted from roots and incubated in hatching dishes. When J2 of Meloidogyne spp. approach roots, their surface coat may be altered, which in turn may reduce their susceptibility to P. penetrans.