Location: Crop Protection and Management ResearchTitle: Comparison of peanut gentics and physical maps provided insights on collinearity, reversions and translocations Author
Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2015
Publication Date: 7/13/2015
Citation: Khera, P., Wang, H., Culbreath, A.K., Khera, P., Kale, S., Pandey, M.K., Varshney, R.K., Wang, J., Guo, B. 2015. Comparison of peanut gentics and physical maps provided insights on collinearity, reversions and translocations. American Peanut Research and Education Society Abstracts. Meeting Abstract. American Peanut Research and Education Society (APRES) meeting, July 14-16, 2015, Charleston, South Carolina. Interpretive Summary:
Technical Abstract: Genetic and physical maps are the valuable resources for peanut research community in understanding genome organization and serving as the basis for map-based cloning and marker-assisted selection. Physical maps of two diploid wild peanut progenitor species, Arachis duranensis (A genome) and A. ipaensis (B genome), have been released in April 2014. Improved version of genetic maps have become available for cultivated tetraploid (AB genome) peanut (A. hypogaea) in last two years. Comparison between the genetic and physical maps, in term of marker positions and orders particularly related to the QTLs, may provide interesting information of the genome structure and marker enrichment in order to produce high-resolution genetic maps. Two genetic maps developed from two recombinant inbred line (RIL) populations, the S population (SunOleic 97R × NC94022) and the T-population (Tifrunner × GT-C20), have been improved with 248 (1425.9 cM) and 426 (1980.8 cM) SSR marker loci. These populations were phenotyped extensively and also were used for detection of quantitative trait loci (QTLs) for disease resistance, oil quality and agronomic traits. Significant collinear relationship was observed in addition to a few reversion and translocations between the genetic and physical maps. For the genetic map generated from S-population, 57% of the markers on the genetic map were mapped on the same pseudomolecules (142 out of 248), while for the T-population, 206 of 426 marker loci (48.3%) were mapped on the same pseudomolecules of the diploid peanut. For example, the major QTLs in the S population were mapped on linkage group (LG) a01 and b03 which had 17 and 16 markers on each LG of the genetic map, respectively, but each had only 10 markers mapped on the corresponding pseudomolecules of the physical map. In the T population, the major QTLs were identified on LGa04, 05 and 06 which had 42, 24, 30 markers on each LG of the genetic map, respectively, but only 22, 15, 18 markers were mapped on the corresponding pseudomolecules of the physical map. The comparison will provide valuable information for improving genetic map resolution, fine mapping the QTLs and map-based cloning of the resistance and other important genes.