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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #316676

Research Project: Developing Soybean and Other Legumes with Resistance to Pathogens and Assessing the Biosafety of Transgenic Soybean

Location: Soybean Genomics & Improvement Laboratory

Title: Soybean cyst nematode, Heterodera glycines, infection assay using soybean roots

Author
item Matthews, Benjamin - Ben
item YOUSSEF, RHEMA - El-Fayoum University

Submitted to: Bio-protocol
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2015
Publication Date: 1/20/2016
Citation: Matthews, B.F., Youssef, R.M. 2016. Soybean cyst nematode, Heterodera glycines, infection assay using soybean roots. Bio-protocol. 6:e1707.

Interpretive Summary: The soybean cyst nematode (SCN) is the most damaging pathogen of soybean. It causes almost $1 billion in soybean yield losses annually in the United States. However, there are no soybean cultivars available that are resistant to all SCN populations. Therefore, much research is being conducted to develop soybean that the farmer can use that is resistant to SCN. Here we describe how to grow, maintain, and harvest SCN. We also describe how to conduct an assay of SCN and how to perform the relevant mathematical calculations to compare one assay with another. This technique is useful to scientists interested in resistance and susceptibility of soybean to SCN.

Technical Abstract: The soybean cyst nematode (SCN) is the most damaging pathogen of soybean. It causes almost $1 billion in soybean yield losses annually in the United States. However, there are no soybean cultivars available that are resistant to all SCN populations. Therefore, much research is being conducted to develop soybean that the farmer can use that is resistant to SCN. Here we describe how to grow, maintain, and harvest SCN. We also describe how to conduct an assay of SCN and how to perform the relevant mathematical calculations to compare one assay with another. This technique is useful to scientists interested in resistance and susceptibility of soybean to SCN.