|Xu, Jingyuan - James|
|TSENG, YIIDER - University Of Florida|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2015
Publication Date: 6/7/2015
Citation: Xu, J., Tseng, Y. 2015. Micro-heterogeneity of corn hulls cellulosic fiber biopolymer studied by multiple-particle tracking (MPT) [abstract]. 28th Annual International Symposium on Polymer Analysis and Characterization (ISPAC 2015). p. 92.
Technical Abstract: A novel technique named multiple-particle tracking (MPT) was used to investigate the micro-structural heterogeneities of Z-trim, a zero calorie cellulosic fiber biopolymer produced from corn hulls. The Multiple-Particle Tracking (MPT) method was used in this study, which was originally described by Apgar et al. . The principle of this technique is to monitor the thermally driven motion of inert micro-spheres, which are evenly distributed within the samples, and to statistically analyze their displacement distributions. From the data of MPT measurements, information about the extent of heterogeneity can be extracted. For each experiment, a dilute suspension of 1 micrometer diameter, fluorescent, polystyrene micro-spheres (0.1 volume percent) was gently mixed with the Z-trim biopolymer. Images of the fluorescent beads were recorded onto the random-access memory of a computer via a cascade 1 k camera mounted on an inverted epifluorescence microscope. Movies were analyzed by a custom MPT routine analysis program from Tseng’s lab. The displacements of the microspheres’ centroids were simultaneously monitored in the focal plane of the microscope for 21.5 seconds at a rate of 30 fps, and the last 20 seconds (total 600 frames) of the movie was taken for particle tracking to avoid the unstable acquisition time in initialization. For each sample of Z-trim, we tracked a total of ~200 microspheres. Individual time-averaged mean squared displacements (MSD),