Location: Sugarbeet and Bean ResearchTitle: Transcriptomes of seeds germinating at temperature extremes Author
|Alzohairy, Safa - Michigan State University|
|Mcgrath, J Mitchell - Mitch|
Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Research Notes
Publication Acceptance Date: 4/15/2015
Publication Date: 6/1/2015
Citation: Alzohairy, S., McGrath, J.M. 2015. Transcriptomes of seeds germinating at temperature extremes. [CD-ROM] 2014 Annual Beet Sugar Development Foundation Research Report. Denver, Colorado: Beet Sugar Development Foundation.
Technical Abstract: Temperature stress on plants is defined as any drop (cold stress) or rise (heat stress) in temperature that causes reversible or irreversible inactivation of physiological processes or lethal injury in plants. In general each plant has an optimum temperature to grow and develop and any deviation than the optimum temperature is considered as temperature stress. Sixty-four East Lansing breeding lines were initially screened for germination at temperature extremes that could be expected under field conditions (e.g. 10 degrees C for early spring planted beets and 40 degrees C for late summer planted beets). RNA was isolated from seeds of two representative germplasms at three temperatures 10, 20 degrees C, or 41 degrees C, sequenced on an Illumina HiSeq 2500 instrument in 150 nt paired end mode. The patterns of gene expression suggests that both heat and cold stress reduce the number of differentially expressed transcripts with similarity to described genes relative to optimal germination conditions in solution.