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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #316235

Research Project: Understanding Genetic and Physiological Factors Affecting Nutrient Use Efficiency of Dairy Cattle

Location: Animal Genomics and Improvement Laboratory

Title: The determination of the concentrations of Isoforms of Vitamin E in tissues, milk and blood via High-Performance Liquid Chromatography (HPLC) after short-term feeding in dairy cows

Author
item Qu, Yang - University Of Maryland
item Elsasser, Theodore
item Newbold, John - Collaborator
item Connor, Erin
item Moyes, Kasey - University Of Maryland

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/2015
Publication Date: 7/12/2015
Citation: Qu, Y., Elsasser, T.H., Newbold, J., Connor, E.E., Moyes, K.M. 2015. The determination of the concentrations of Isoforms of Vitamin E in tissues, milk and blood via High-Performance Liquid Chromatography (HPLC) after short-term feeding in dairy cows. Meeting Abstract. 93(Suppl. 2):573.

Interpretive Summary:

Technical Abstract: The objective of this study was to determine the pattern of change in the concentrations of the four isoforms of vitamin E (alpha-, beta-, gamma- and delta-tocopherol) in bovine tissues (liver, mammary and muscle), blood and milk after short-term feeding of a vegetable-derived oil (Tmix) particularly enriched with gamma- and delta-isoforms (9% alpha-, 1% beta-, 24% delta- and 62% gamma-tocopherol). Healthy multiparous Holstein cows (> 90 DIM) were assigned to dietary control (Notoc, n=4) or tocopherol-fed treatment groups (n=5: TOC;~260g Tmix/cow/d, top-dressed) for 9 consecutive days. Milk and blood samples were collected in the mornings on d 0 and d 10 from the 5 TOC cows; tissues were harvested from those same cows on d 10 of treatment and the cohort of physiologically similar Notoc cows. Isoform concentrations were determined in samples by HPLC. Data were analyzed by Student’s t-test and ANOVA. Delta-TOC was not detected in tissues, blood or milk. Concentrations of gamma-TOC were increased (P<0.001) in tissues, blood and milk with feeding (P<0.001). In all tissues, alpha-TOC concentrations were higher (P<0.001) than gamma-TOC before and after feeding. Concentrations of both alpha- and gamma-TOC were higher in blood and milk after feeding Tmix (P<0.05). The content of alpha-TOC was higher than gamma-tocopherol (P<0.001) in milk and blood before and after feeding. In conclusion, short-term feeding of Tmix oil resulted in increases in the gamma-isoform, largely no measurable content or significant change in the beta- or delta-isoforms, and a variable change in the alpha-isoform across various tissues and biological fluids of the cow.