|ZHENG, YI - Boyce Thompson Institute|
|FEI, ZHANGJUN - Boyce Thompson Institute|
Submitted to: Genome Announcements
Publication Type: Research Notes
Publication Acceptance Date: 4/3/2015
Publication Date: 5/7/2015
Publication URL: http://doi:10.1128/genomeA.00452-15
Citation: Li, R., Zheng, Y., Fei, Z., Ling, K. 2015. First complete genome sequence of an emerging cucumber green mottle mosaic virus isolate in North America. Genome Announcements. 3(3):300452-15.
Interpretive Summary: Cucumber green mottle mosaic virus (CGMMV), an emerging virus infecting greenhouse cucumber, was first reported in North America in 2013. In the present study, the complete genome sequence of the original Canadian CGMMV isolate was determined. Sequence analysis demonstrated that this Canadian CGMMV isolate shares a strong (99%) genome sequence identity with CGMMV isolates from Asia, but only a 90% identity to isolates from Europe. This strong genetic relationship with the Asian genotype suggests that this seed-borne virus may have been introduced to Canada from a contaminated seed lot that was originally produced in Asia.
Technical Abstract: The complete genome sequence (6,423 nt) of an emerging Cucumber green mottle mosaic virus (CGMMV) isolate on cucumber in North America was determined through deep sequencing of sRNA and rapid amplification of cDNA ends. It shares 99% nucleotide sequence identity to the Asian genotype, but only 90% to the European genotype.