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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #315638

Research Project: Using Genetic Approaches to Reduce Crop Losses in Rice Due to Biotic and Abiotic Stress

Location: Dale Bumpers National Rice Research Center

Title: Development of molecular markers for breeding for disease resistant crops

item Jia, Yulin
item LIU, YAN - University Of Arkansas
item WANG, XUEYAN - University Of Arkansas
item SCHEUERMANN, KLAUS - University Of Santa Catarina
item Jia, Melissa
item McClung, Anna

Submitted to: International Plant Protection Congress
Publication Type: Proceedings
Publication Acceptance Date: 4/28/2015
Publication Date: 8/24/2015
Citation: Jia, Y., Liu, Y., Wang, X., Scheuermann, K., Jia, M.H., Mcclung, A.M. 2015. Development of molecular markers for breeding for disease resistant crops. Permanent identification: urn:nbn:de:0294-sp-2015-1-2. Proceedings of XVIII International Plant Protection Congress. August 24-28, 2015. Berlin, Germany. OBT3, Page 88.

Interpretive Summary:

Technical Abstract: Rice blast disease caused by the filamentous ascomycetes fungus Magnaporthe oryzae and sheath blight disease caused by the soil borne fungus Rhizocotonia solani are the two major rice diseases that threaten stable rice production in the USA and worldwide. These two diseases have been managed with a combination of resistance (R) genes and fungicide application integrated into diverse culture practices worldwide. Application of fungicides adds additional production costs and excessive fungicide application brings increased environmental concern. Using R genes is the most economical and environmentally benign method for crop protection. In an effort to aid classical plant breeding for improving resistance to blast and sheath blight diseases, we have been developing DNA markers for use in marker assisted breeding. These molecular markers were derived from closely linked DNA markers to major or minor R genes, portions of cloned R genes by association analysis of disease reactions with DNA sequence variation of candidate genes, or cloned R genes in mapping populations and selected rice germplasm. Consequently, USA rice cultivars have been developed with enhanced disease resistance using these markers. Our current effort for DNA marker development using improved disease assays, and genotyping by sequencing (GBS) using next gen sequencing will be presented.