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Title: Effect of in-feed supplementation of trans-cinnamaldehyde and caprylic acid on chicken cecal microbiome in response to Salmonella Enteritidis

item UPADHYAYA, INDU - University Of Connecticut
item CHEN, CHI-HUNG - University Of Connecticut
item UPADHYAY, ABHINAV - University Of Connecticut
item YIN, HSIN-BAI - University Of Connecticut
item NAIR, MEERA - University Of Connecticut
item KHAN, M - University Of Connecticut
item DARRE, MICHAEL - University Of Connecticut
item Donoghue, Ann - Annie
item DONOGHUE, DAN - University Of Arkansas
item VENKITANARAYAMNAN, KUMAR - University Of Connecticut

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/26/2015
Publication Date: 7/27/2015
Citation: Upadhyaya, I., Chen, C., Upadhyay, A., Yin, H., Nair, M.S., Khan, M.I., Darre, M.J., Donoghue, A.M., Donoghue, D.J., Venkitanarayamnan, K. 2015. Effect of in-feed supplementation of trans-cinnamaldehyde and caprylic acid on chicken cecal microbiome in response to Salmonella Enteritidis. [abstract]. Poult. Sci. 94:59 (E-Suppl. 1).

Interpretive Summary:

Technical Abstract: Salmonella Enteritidis (SE) is a major foodborne pathogen causing enteric illnesses in humans, with undercooked eggs and poultry meat as the primary sources of infection. Our previous research revealed that in-feed supplementation of two GRAS (generally recognized as safe)-status, natural compounds, namely trans- cinnamaldehyde (TC, from cinnamon) and caprylic acid (CA, from goat milk) significantly reduced cecal colonization and egg-borne transmission of SE in chickens. In this study, the effect of in-feed supplementation of TC and CA on the cecal microbiome of chickens was determined in two separate trials using 24- and 40-wk-old birds. In each trial, 120 single-comb White Leghorn hens were randomly assigned to six treatments (n=20/treatment): a negative control (no SE, no compound), two compound controls (no SE, 1% vol/wt TC or 1% vol/wt CA), a positive control (SE, no TC or CA), TC treatment (SE, 1% TC) and CA treatment (SE, 1% CA). On day 0, the birds were tested for any inherent Salmonella (n=3/experiment), and the compounds were supplemented in the feed for 60 days. On day 10, birds in the positive controls, TC and CA treatments were challenged with SE (10 log10 CFU/bird) by crop gavage. Cecal contents were collected from birds on day 0, 1, 7, 10, 20, 30 and 60 days and the contents were subjected to 16S rRNA sequencing by Illumina Miseq. Results suggest that there was no adverse effect on the population of the major bacterial phylotypes in the chicken cecum, including Firmicutes, Bacteroidetes and Proteobacteria (P>0.05). Additionally, TC and CA supplementation reduced SE in the cecum, yolk and eggshell of birds when compared to controls (P<0.05). The results indicate that TC and CA could be effectively used as feed additives to reduce SE colonization in chickens without deleteriously affecting the endogenous cecal microflora of birds. Funded in part by the USDA-NIFA-OREI 2011-01955.