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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #315388

Research Project: Alternative Intervention and Control Strategies for Foodborne Pathogens in Poultry and Poultry Products

Location: Poultry Production and Product Safety Research

Title: The ability of select probiotics to reduce enteric Campylobacter colonization in broiler chickens

Author
item Shrestha, Sandip - University Of Arkansas
item Donoghue, Ann - Annie
item Arsi, Komala - University Of Arkansas
item Woo-ming, Ann - University Of Arkansas
item Wagle, Basanta - University Of Arkansas
item Blore, Pamela - University Of Arkansas
item Donoghue, Dan - University Of Arkansas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/26/2015
Publication Date: 7/27/2015
Citation: Shrestha, S., Donoghue, A.M., Arsi, K., Woo-Ming, A., Wagle, B.R., Blore, P., Donoghue, D. 2015. The ability of select probiotics to reduce enteric Campylobacter colonization in broiler chickens. [abstract]. Poult. Sci. 94:44 (E-Suppl. 1).

Interpretive Summary:

Technical Abstract: Campylobacter is the leading cause of foodborne illness worldwide and is often associated with consumption and/or mishandling of contaminated poultry products. Probiotic use in poultry has been an effective strategy in reducing other enteric foodborne pathogens but not consistently for Campylobacter. As Campylobacter resides and utilizes intestinal mucin for growth, isolates selected on the basis of mucin utilization might be a strategy to screen for efficacious probiotic bacterium. In this study, bacterial isolates demonstrating increased growth rates in the presence of mucin in broth were tested in a total of four bird trials. In both trials 1 and 2, ninety day-of-hatch chicks were randomly divided into 9 treatment groups (n=10/treatment) and treated individually with one of four bacterial isolates (Bacillus sp.) grown in media with or without mucin prior to inoculation or a Campylobacter positive control (no probiotics). In trials 3 and 4, sixty day-of-hatch chicks were divided into 6 treatment groups (n=10/treatment) and were dosed with five individual isolates (Lactobacillus sp.) all grown in mucin prior to inoculation or a Campylobacter positive control (no probiotic). All birds were gavaged with individual isolates at day-of-hatch and orally challenged with a four strain mixture C. jejuni on day 7. Ceca were collected at day 14 for Campylobacter enumeration. Campylobacter counts were logarithmically transformed (log10 CFU/g) and treatment means were partitioned by LSMEANS analysis (P < 0.05). Results from these trials demonstrated two individual isolates grown in mucin prior to inoculation consistently reduced cecal Campylobacter counts (1-2 log reduction). These results support the potential use of preselection and growth of isolates in mucin in evaluating bacterial isolates with the ability to reduce enteric Campylobacter colonization. Funded in part by the USDA-NIFA-OREI 2011-01955.