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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #314672

Research Project: Genetic Improvement of Hard Winter Wheat to Biotic and Abiotic Stresses

Location: Hard Winter Wheat Genetics Research

Title: Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS

Author
item SHEN, X.K. - Sichuan University
item MA, L.X. - Sichuan University
item ZHONG, S.F. - Sichuan University
item LIU, NA - Sichuan University
item ZHANG, M. - Sichuan University
item CHEN, W.Q. - Sichuan University
item ZHOU, Y.L. - Sichuan University
item LI, H.J. - Beijing Academy Of Agricultural Sciences
item ZHANG, Z.J. - Shanxi University
item LI, X - Sichuan University
item Bai, Guihua
item ZHANG, H.Y. - Sichuan University
item TAN, F.Q. - Sichuan University
item REN, Z.L. - Sichuan University
item LUO, P.G. - Sichuan University

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/17/2014
Publication Date: 1/4/2015
Citation: Shen, X., Ma, L., Zhong, S., Liu, N., Zhang, M., Chen, W., Zhou, Y., Li, H., Zhang, Z., Li, X., Bai, G., Zhang, H., Tan, F., Ren, Z., Luo, P. 2015. Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS. Theoretical and Applied Genetics. doi: 10.1007/s00122-014-2449-x.

Interpretive Summary: Powdery mildew (PM) is a destructive foliar disease in wheat. To improve wheat resistance to PM, a chromosome fragment from a wheat wild relative, Thinopyrum intermedium, was transferred to a Chinese common wheat line, L962. The alien fragment confers resistance to multiple Chinese PM isolates. Genetic analysis using the F1, F2, and F2:3 populations derived from the cross of L983/L962 indicated that resistance was controlled by a single dominant gene. This gene was mapped to the short arm of chromosome 2B and is a novel PM resistance gene designated as PmL962. Two flanking markers to the gene, Xwmc314 and BE443737 at 5.83 cM apart, were identified. The flanking markers have potential applications in marker-assisted breeding.

Technical Abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting the production of wheat (Triticum aestivum). Powdery mildew resistance was putatively transferred from Thinopyrum intermedium to the common wheat line L962, which conferred resistance to multiple Chinese Bgt isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F1, F2, and F2:3 populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F2:3 lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated PmL962. It is flanked by Xwmc314 and BE443737 at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in Brachypodium distachyon and a 223.5 kb genomic region on rice (Oryza sativa) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding.