Location: Renewable Product Technology ResearchTitle: Pilot-scale bioreactor production and long term stability of feruloyl soy glycerides Author
|Compton, David - Dave|
|Goodell, John - Biotechnology Research And Development Corporation (BRDC)|
|Grall, Steven - Food Plant Design, Llc|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/20/2015
Publication Date: 8/20/2015
Citation: Compton, D.L., Goodell, J.R., Grall, S., Evans, K.O. 2015. Pilot-scale bioreactor production and long term stability of feruloyl soy glycerides. American Chemical Society. AGFD 165.
Technical Abstract: Soybean oil was transesterified with ethyl ferulate at 60 °C using the immobilized lipase Candida antartica lipase B (Novozym 435) to produce a mixture of feruloylated monoacylglycerols and feruloylated diacylglycerols homologues, collectively referred to as feruloylated soy glycerides (FSG). A 1-tonne (1,000 kg)/yr, continuous, packed-bed bioreactor was designed and implemented for production of FSG. The substrate was fed to a series of four 10-L, packed bed columns containing Novozym 435 for over five continuous months. The FSG yield was ~ 65% over the course of the 450 kg production run with no loss of enzyme activity. FSG physicochemical characteristics from the most recent production run were compared to FSG produced five years ago to assess FSG stability. Antioxidant capacity (DPPH assay), oxidative stability (iodine number), and susceptibility to hydrolysis (Karl Fischer titration, total acid number, Lovibond color) were compared.