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Research Project: Strategies to Control and Prevent Avian Mycoplasmosis

Location: Poultry Research

Title: Proteomic analysis of Mycoplasma gallisepticum vaccine strain F

Author
item Collier, Stephanie
item Branton, Scott
item Evans, Jeff
item Leigh, Spencer
item Wan, X-f - Mississippi State University
item Cooksey, A - University Of Arizona
item Olanrewaju, Hammed
item Pharr, G - Mississippi State University

Submitted to: International Journal of Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/25/2015
Publication Date: 5/5/2015
Citation: Collier, S.D., Branton, S.L., Evans, J.D., Leigh, S.A., Wan, X., Cooksey, A.M., Olanrewaju, H.A., Pharr, G.T. 2015. Proteomic analysis of Mycoplasma gallisepticum vaccine strain F. International Journal of Poultry Science. 14(1): 1-12.

Interpretive Summary: The Mycoplasma gallisepticum vaccine F-strain (F-strain) has been shown to persist in the host as well as to displace virulent strains of Mycoplasma gallisepticum (MG). Understanding the mechanism(s) of colonization and persistence of F-strain will aid in the current intervention strategies to diagnose and control MG infections in poultry. In the present study, phase partition and liquid chromatography along with electrospray mass spectrometry were used to evaluate the proteome of F-strain. A total of 586, 478 and 339 proteins were recognized from whole cell lysate (total protein), aqueous (cytosolic) and detergent phases (membrane), respectively. The proteins found among the total proteins identified using database searches were then grouped into three categories: (1) proteins from the membrane phase (TM); (2) proteins from the cytosolic phase (TC); and (3) proteins derived from the membrane and cytosolic phases (TMC). There were a total of 93 (33 as putative membrane proteins predicted by SOSUI), 207(13) and 79(6) proteins in the TM, TC and TMC, respectively. The identified proteins were distributed among John Craig Venter Institute categories, with the majority predicted to be involved in protein synthesis. Investigation of the proteome of F-strain my aid in the identification and characterization of F-strain proteins that are important in host colonization.

Technical Abstract: The persistence and displacement abilities of the Mycoplasma gallisepticum vaccine strain F (F-strain) are well documented. Understanding the mechanism(s) of colonization and persistence of F-strain will aid in the current intervention strategies to diagnose and control MG infections in poultry. In the present study, phase partition and liquid chromatography along with electrospray mass spectrometry were used to evaluate the proteome of F-strain. A total of 586, 478 and 339 proteins were recognized from whole cell lysate (total protein), aqueous (cytosolic) and detergent phases (membrane), respectively. The proteins identified with the database searches were then grouped into three categories: (1) proteins from the membrane phase and found in the total proteins (TM); (2) proteins from the cytosolic phase and found in the total proteins (TC); and (3) proteins derived from the membrane and cytosolic phases and found in the total proteins (TMC). There were a total of 93 (33 as putative membrane proteins predicted by SOSUI), 207(13) and 79(6) proteins in the TM, TC and TMC, respectively. The identified proteins were distributed among John Craig Venter Institute (JCVI) categories, with the majority predicted to be involved in protein synthesis. Investigation of the Mycoplasma gallisepticum F-strain proteome may aid in the identification and characterization of F-strain proteins that are important in host colonization.