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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety & Processing Research » Research » Publications at this Location » Publication #313645

Research Project: Pathogen Reduction and Processing Parameters in Poultry Processing Systems

Location: Poultry Microbiological Safety & Processing Research

Title: Ability of Cecal Cultures to Inhibit Growth of Salmonella Typhimurium during Aerobic Incubation

Author
item Hinton, Jr, Arthur
item Gamble, Gary
item Ingram, Kimberly - Kim
item Holser, Ronald

Submitted to: International Association for Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: 3/19/2015
Publication Date: 6/25/2015
Citation: Hinton Jr, A., Gamble, G.R., Ingram, K.D., Holser, R.A. 2015. Ability of Cecal Cultures to Inhibit Growth of Salmonella Typhimurium during Aerobic Incubation [abstract]. International Association for Food Protection. (Suppl.A):69.

Interpretive Summary:

Technical Abstract: Introduction: Poultry can serve as reservoirs for Salmonella; however, chicks provided cultures of cecal bacteria develop resistance to colonization by Salmonella. Research has indicated that cecal bacteria metabolize organic acids to produce substances that inhibit Salmonella growth. Purpose: The ability of cecal bacterial cultures to inhibit Salmonella growth when incubated aerobically in media supplemented with lactate and succinate was examined, and bacteria in the cultures were identified. Methods: Cecal cultures were prepared by inoculating broth media with cecal contents of broilers and incubating media anaerobically at 35oC for 48 h. Media supplemented with 0, 50, 100, or 150 mM of lactate and succinate were inoculated with 0.1 ml of the cecal culture, 104 cfu/ml of Salmonella Typhimurium, or the cecal culture and Salmonella. Inoculated media were incubated aerobically at 35oC for 14 days, and aliquots of media were removed on Days 0, 7, and 14 to enumerate cfu/ml (n = 3). Results: After 14 days of incubation of media inoculated with Salmonella only, there were significant (P < 0.05) increases in the number of Salmonella recovered from media supplemented with 0, 50, 100, or 150 mm of lactate and succinate. However; after incubation of media inoculated with Salmonella and cecal cultures there was no significant increase in the number of Salmonella recovered from media that was not supplemented with lactate and succinate, while there were significant decreases in the number of Salmonella recovered from media supplemented with 50, 100, or 150 mm of lactate and succinate. Enterococcus, Bacillus, and Proteus were recovered from the media. Significance: Findings indicate that cecal cultures incubated aerobically can metabolize lactate and succinate to produce substances that inhibit the growth of Salmonella. Identifying the bacteria that produce these substances will aid the formulation of defined probiotics that can reduce the colonization of broilers by Salmonella.