Location: Southern Horticultural ResearchTitle: Screening for anthracnose disease resistance in strawberry using a detached leaf assay
|Miller Butler, Melinda|
|CURRY, K - University Of Southern Mississippi|
|KREISER, B - University Of Southern Mississippi|
|BLYTHE, E - Mississippi State University|
Submitted to: North American Strawberry Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/2015
Publication Date: 1/29/2015
Citation: Miller Butler, M.A., Curry, K.J., Kreiser, B.R., Smith, B.J., Blythe, E.K. 2015. Screening for anthracnose disease resistance in strawberry using a detached leaf assay. North American Strawberry Conference Abstracts. p. 19.
Technical Abstract: Inoculation of detached strawberry leaves with Colletotrichum species may provide a rapid, non-destructive method of identifying anthracnose resistant germplasm. The reliability and validity of assessing disease severity is critical to disease management decisions. We inoculated detached strawberry leaves with Colletotrichum fragariae and C. gloeosporioides from named cultivars (n = 10) and C. fragaria resistant clones (n = 10). We then determined anthracnose disease severity with a quantitative assessment made via computer based image analysis and visual assessments made by two independent raters. The image analysis provided a precise measurement of percent lesion area of infected leaves and we used this to establish a “gold standard” upon which to compare visual disease assessments. Visual assessments (n = 944) were compared to each other and the inter-rater reliability strength of agreement was good (Weighted Kappa = 0.82; 95% CI: 0.79-0.84). Detached leaf assays may provide a reliable method for resistance screening.