Submitted to: Journal of Wound Repair and Regeneration
Publication Type: Abstract Only
Publication Acceptance Date: 1/2/2015
Publication Date: 6/17/2015
Citation: Edwards, J.V., Prevost, N.T., Condon, B.D., Gary, L. 2015. Low level hydrogen peroxide generation from a nonwoven fibrous pectin-cellulose blend. Journal of Wound Repair and Regeneration. 23(2):A20.
Technical Abstract: Fibrous pectic-cellulose (FPC) (cellulose blended with primary cell wall pectin at 2 percent by weight of pectin) is product made from naturally occurring plant fibers. FPC is a fibrous mixture of polysaccharides with a low percent by weight of pectin-based primary cell wall and lipid components attached to absorbent cellulose I (microfibrillar bundles). FPC can be hydroentangled into nonwoven materials through a process that retains these constituents and has suitable structural properties for semi-occlusive wound dressings. FPC has been found to generate low-level hydrogen peroxide. Low level hydrogen peroxide is associated with cell signaling leading to enhanced granulation tissue and it may give rise to improved cell proliferation and fibroblast and macrophage production in chronic wounds. Here several varieties of FPC are examined for levels of pectin, primary cell wall enzymes, and metals present in the plant derived material associated with hydrogen peroxide generation. Pectin was analyzed by hydrolyzing FPC with Viscozyme, and a colorimetric procedure in which uronic acids are quanti?ed spectrophotometrically at pH 4.8. Amplex Red reagent was employed to measure H2O2. Metals were measure by ICP, and Superoxide Dismutase assays were employed. FPC levels of hydrogen peroxide generation varied from 0.2 – 24 micromolar based on the origin and preparation. Pectin levels varied from 40-100 micrograms/gram, and SOD activity varied from 0.2 – 2.5 U/mL. Levels of calcium (~400 micrograms/gram), copper (1.3 micrograms/gram) and iron (28 micrograms/gram) that influence superoxide formation were retained at varying levels in the nonwoven material. The role of these in the mechanism of hydrogen peroxide generation is discussed. This work demonstrates the potential to develop FPC into a nonwoven dressing that can generate low level hydrogen peroxide commensurate with the range found to enhance fibroblast cell proliferation.