|HATMAKER, E. ANNE - University Of Tennessee|
|WADL, PHILLIP - University Of Tennessee|
|MANTOOTH, KRISTIE - University Of Tennessee|
|OWNLEY, BONNIE - University Of Tennessee|
|TRIGIANO, ROBERT - University Of Tennessee|
Submitted to: Applications in Plant Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/5/2015
Publication Date: 4/7/2015
Publication URL: http://handle.nal.usda.gov/10113/60648
Citation: Hatmaker, E., Wadl, P.A., Mantooth, K., Scheffler, B.E., Ownley, B.H., Trigiano, R.N. 2015. Development of microsatellites from Fothergilla xintermedia (Hamamelidaceae) and cross transfer to four other genera within Hamamelidaceae. Applications in Plant Sciences. 3(4):1400123. doi.org/10.3732/apps.1400123. Availiable: http://www.bioone.org/doi/full/10.3732/apps.1400123.
Interpretive Summary: Fothergilla is a genus of deciduous flowering shrub species in the Hamamelidaceae that are native to the southeastern United States (US). Fothergilla major is indigenous to the Appalachian Mountains and southeastern piedmont regions, whereas F. gardenii, is found throughout the coastal plains (Weakley, 2006). Fothergilla major is a threatened species in Tennessee whereas F. gardenii is threatened in Georgia and endangered in Florida (USDA, 2012). Hamamelis species are found in the eastern US, Mexico, China and Japan (Zhiyun et al., 2003). The genus includes six species, with four “major species” recognized by Marquard et al. (1997), whereas Xie et al. (2010) recognized the following six species: H. vernalis, H. mexicana, H. virginiana, H. mollis, H. japonica, and H. ovalis. Hamamelis mollis is found in China, H. japonica in Japan, H. mexicana in Mexico, and H. ovalis is found in a small section of Mississippi, whereas H. vernalis and H. virginiana have a wider range throughout eastern North America. The basis for this work was the development of DNA markers for the characterization of Fothergilla accessions and related species. Markers were isolated, validated and characterized on Fothergilla and related species.
Technical Abstract: Premise of the study: Develop microsatellites from Fothergilla ×intermedia to establish loci capable of distinguishing species and cultivars, and assess genetic diversity for use by ornamental breeders, and for transfer within Hamamelidaceae. Methods and Results: A small insert genomic library enriched for microsatellites was sequenced to develop 12 polymorphic microsatellite loci. The number of alleles detected ranged from 4 to 9 across 5 genera within Hamamelidaceae. Shannon’s information index ranged from 0.9 to 0.13. Conclusions: These microsatellite loci provide a set of markers to evaluate genetic diversity of natural and cultivated collections and assist ornamental plant breeders for five popular genera of woody ornamental plants.