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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Residue Chemistry and Predictive Microbiology Research » Research » Publications at this Location » Publication #313031

Research Project: DEVELOPMENT OF PREDICTIVE MICROBIAL MODELS FOR FOOD SAFETY AND THEIR ASSOCIATED USE IN INTERNATIONAL MICROBIAL DATABASES

Location: Residue Chemistry and Predictive Microbiology Research

Title: Rapid detection and differentiation of Listeria monocytogenes and Listeria species in deli meats by a new multiplex PCR method

Author
item Iu, Haiquan - Shanghai University
item Lu, Liqun - Shanghai University
item Pan, Yingjie - Shanghai University
item Sun, Xiaohong - Shanghai University
item Hwang, Cheng-an - Andy
item Zhao, Yong - Shanghai University
item Wu, Vivian - University Of Maine

Submitted to: Food Control
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/14/2014
Publication Date: 6/1/2015
Citation: Iu, H., Lu, L., Pan, Y., Sun, X., Hwang, C., Zhao, Y., Wu, V.C. 2015. Rapid detection and differentiation of Listeria monocytogenes and Listeria species in deli meats by a new multiplex PCR method. Food Control. 52:78-84.

Interpretive Summary: Listeria monocytogenes is a foodborne pathogen capable of causing severe illnesses. It is necessary to develop a method that can differentiate L. monocytogenes from other Listeria species in food and environmental samples. A new multiplex PCR method using new primers targeting the iap gene of Listeria for detecting L. monocytogenes was developed. The new PCR method was able to differentiate L. monocytogenes from other Listeria species of type cultures and isolates of meat samples. This simple method can be used as one measure to reduce L. monocytogenes contamination in food products.

Technical Abstract: Listeria monocytogenes is an important foodborne pathogen. To effectively control this pathogen, it is necessary to have a method that can detect and differentiate L. monocytogenes from other Listeria species in food, environmental, and clinical samples. A new multiplex PCR method using new primers targeting the iap gene was developed to detect and differentiate L. monocytogenes and other Listeria species, and the method was applied to examine deli meat samples. Sixteen Listeria strains (8 L. monocytogenes, 2 L. innocua, 1 L. seeligeri, 3 L. welshimeri, 1 L. ivanovii, and 1 L. grayi) from the American Type Culture Collection were tested using the new PCR method in comparison to 16S rRNA sequencing assay and a previously described PCR method based on the detection of iap gene with other primers. The new PCR method was able to differentiate L. monocytogenes from the other Listeria species, while the 16S rRNA sequencing assay could not distinguish L. welshimeri from L. monocytogenes and the previously published multiplex PCR assay was not able to distinguish L. monocytogenes from L. innocua or L. Ivanovii. Furthermore, 150 deli meat samples were analyzed by the newly developed multiplex PCR in comparison to ISO11290-1 method and 16S rRNA sequencing assay to detect L. monocytogenes and differentiate it from the other 5 species of Listeria. The developed PCR provided the same results as the ISO method. Out of 150 samples, 16 (10.7%) samples and 5 (3.3%) samples were tested positive and confirmed for Listeria spp. and L. monocytogenes, respectively. The multiplex PCR method is capable of discriminating between L. monocytogenes and other Listeria species. The method is simple and specific when combined with simplified enrichment procedures.