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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Publications at this Location » Publication #312829

Research Project: CEREAL RUST FUNGI: GENETICS, POPULATION BIOLOGY, AND HOST-PATHOGEN INTERACTIONS

Location: Cereal Disease Lab

Title: Collections of Puccinia triticina in different provinces of China are highly related for virulence and molecular genotype

Author
item Kolmer, James - Jim

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/7/2015
Publication Date: 5/1/2015
Citation: Kolmer, J.A. 2015. Collections of Puccinia triticina in different provinces of China are highly related for virulence and molecular genotype. Phytopathology. 105:700-706.

Interpretive Summary: Wheat is attacked by Puccinia triticina, which is the scientific name of the fungus that causes the disease wheat leaf rust. This disease occurs world wide. The purpose of this study was to genetically characterize the P. triticina population in China using DNA based molecular markers and also by testing the P. triticina isolates for their ability to attack different resistance genes in wheat. Eighty-one isolates of P. triticina from China were placed into three genetically distinct groups. The three groups were widley distributed across China. The three groups also differed for abililty to attack important leaf rust resistance genes in wheat. The DNA patterns of the isolates from China will be used to compare P. triticina populations in the U.S. and world wide to determine how this fungus migrates around the world, and to determine the origin of any new biotypes of the fungus that may be found in the U.S.

Technical Abstract: Collections of Puccinia triticina, the wheat leaf rust pathogen, were obtained from seven provinces in China from 2009 and 2010. Single uredinial isolates were derived and tested for virulence phenotype to 20 lines of Thatcher wheat that differ for single leaf rust resistance genes, and for molecular genotype with 23 simple sequence repeat (SSR) primers. Forty-eight virulence phenotypes were described among the 155 isolates tested for virulence. All but four isolates were virulent to Lr26, and no isolates with virulence to Lr18 or Lr24 were found. The three most common phenotypes, FCBQQ, PCGLN, and PCGLL were found in five, five, and three provinces, respectively. Eighty-one SSR genotypes were found among the 100 isolates tested for molecular variation. Isolates with identical virulence phenotypes and SSR genotypes were found in more than one province. Analysis of variation showed no overall differentiation of SSR genotypes or virulence phenotypes based on province of origin. The SSR genotypes had high levels of linkage disequilibrium, high levels of observed heterozygosity, and significant correlation with the virulence phenotypes, all measures that indicated clonal reproduction. Bayesian cluster analysis and principle component plots indicated three groups of SSR genotypes that also varied significantly for virulence. The seven provinces are continuously adjacent to each other and likely form a single epidemiological zone for P. triticina.