Submitted to: International Journal of Food Microbiology
Publication Type: Review Article
Publication Acceptance Date: 5/29/2015
Publication Date: 11/20/2015
Publication URL: http://handle.nal.usda.gov/10113/62346
Citation: Kurtzman, C.P. 2015. Identification of food and beverage spoilage yeasts from DNA sequence analyses. International Journal of Food Microbiology. 213:71-78.
Technical Abstract: Detection, identification, and classification of yeasts has undergone a major transformation in the last decade and a half following application of gene sequence analyses and genome comparisons. Development of a database (barcode) of easily determined DNA sequences from domains 1 and 2 (D1/D2) of the nuclear large subunit rRNA gene and from ITS now permits many laboratories to identify species quickly and accurately, thus replacing the laborious and often inaccurate phenotypic tests previously used. Phylogenetic analysis of gene sequences has resulted in a major revision of yeast systematics resulting in redefinition of nearly all genera. This new understanding of species relationships has prompted a change of rules for naming and classifying yeasts and other fungi, and these new rules are presented in the recently implemented International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). The use of molecular methods for species identification and the impact of Code changes on classification will be discussed, especially in the context of food and beverage spoilage yeasts.