|Weaver, J. Todd - Animal And Plant Health Inspection Service (APHIS)|
|Malladi, Sasidhar - University Of Minnesota|
Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 1/3/2015
Publication Date: N/A
Technical Abstract: Lateral flow devices (LFD) are commercially available and provide a fast, highly specific, on-site test for avian influenza. Because of the low analytic sensitivity of LFD tests at low virus concentrations, targeted sampling of sick and dead birds has been proposed in order to increase detection probability. Three experiments were conducted to characterize the performance of these tests using low and high dose exposure to an H7N3 HPAIV with a short mean death time (MDT) and low dose exposure to an H5N2 highly pathogenic avian influenza virus (HPAIV) with a long MDT. Oropharyngeal swab samples were collected at 12hr intervals from 12 through 108 hours post inoculation and were tested immediately with the 2 LFD commercially available in the U.S. Clinical conditions of chickens were recorded and virus titers were determined by quantitative real-time reverse transcription-polymerase chain reaction. Also, 50% of the dead birds were held an additional 12 hours prior to testing, to mimic field conditions where birds may not be tested immediately, to determine if there was any impact on testing.